Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14892 and 14893 are separated by 282 nucleotides14893 and 14894 are separated by 965 nucleotides14894 and 14895 are separated by 214 nucleotides14895 and 14896 are separated by 198 nucleotides mRNA_6524: 14892 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SUPERFAMILY--SSF81383, at 999,531 to 1,001,982 14892 mRNA_6525: 14893 - HMMPfam-WD domain, G-beta repeat-PF00400,ProSiteProfiles-Trp-Asp (WD) repeats profile.-PS50082,ProSiteProfiles-Trp-Asp (WD) repeats circular profile.-PS50294,ProSiteProfiles-LIS1 homology (LisH) motif profile.-PS50896,SMART-WD40 repeats-SM00320,SUPERFAMILY--SSF50978, at 1,002,265 to 1,003,923 14893 mRNA_6526: 14894 - KOG2929 Transcription factor, component of CCR4 transcriptional complex, at 1,004,889 to 1,006,380 14894 mRNA_6527: 14895 - K18178 COA5, PET191 cytochrome c oxidase assembly factor 5, at 1,006,595 to 1,007,064 14895 mRNA_6528: 14896 - K01514 PRUNE, PPX1 exopolyphosphatase, at 1,007,263 to 1,008,621 14896
Group Condition mRNA_6524 mRNA_6525 mRNA_6526 mRNA_6527 mRNA_6528
14892 14893 14894 14895 14896
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.6 N.D. N.D. -1.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -1.2 N.D. N.D. -0.9
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.1 N.D. N.D. -1.9
growth on ricinoleic acid YNB Glucose -0.1 -0.8 N.D. N.D. -0.7
growth on oleic acid YNB Glucose +0.1 -0.1 N.D. N.D. -1.4
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.2 N.D. N.D. -1.2
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.2 N.D. N.D. -0.3
growth on oleic acid YNB Oleic Acid +0.2 -0.4 N.D. N.D. -0.2
growth on ypd YPD +0.5 +0.4 N.D. N.D. -1.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.4 N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.1 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.3 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.3 N.D. N.D. -0.3
auxotrophy Dropout Complete +0.5 +0.3 N.D. N.D. -0.9
auxotrophy YNB Glucose +0.4 -0.0 N.D. N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.3 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.1 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.5 +0.5 N.D. N.D. -0.6
auxotrophy YNB Glucose 75mM Methionine +0.7 +0.3 N.D. N.D. -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.3 N.D. N.D. +1.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.1 N.D. N.D. +1.2
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