Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14893 and 14894 are separated by 965 nucleotides14894 and 14895 are separated by 214 nucleotides14895 and 14896 are separated by 198 nucleotides14896 and 14897 are separated by 297 nucleotides mRNA_6525: 14893 - HMMPfam-WD domain, G-beta repeat-PF00400,ProSiteProfiles-Trp-Asp (WD) repeats profile.-PS50082,ProSiteProfiles-Trp-Asp (WD) repeats circular profile.-PS50294,ProSiteProfiles-LIS1 homology (LisH) motif profile.-PS50896,SMART-WD40 repeats-SM00320,SUPERFAMILY--SSF50978, at 1,002,265 to 1,003,923 14893 mRNA_6526: 14894 - KOG2929 Transcription factor, component of CCR4 transcriptional complex, at 1,004,889 to 1,006,380 14894 mRNA_6527: 14895 - K18178 COA5, PET191 cytochrome c oxidase assembly factor 5, at 1,006,595 to 1,007,064 14895 mRNA_6528: 14896 - K01514 PRUNE, PPX1 exopolyphosphatase, at 1,007,263 to 1,008,621 14896 mRNA_6529: 14897 - K03626 EGD2, NACA nascent polypeptide-associated complex subunit alpha, at 1,008,919 to 1,009,693 14897
Group Condition mRNA_6525 mRNA_6526 mRNA_6527 mRNA_6528 mRNA_6529
14893 14894 14895 14896 14897
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. -1.9 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid -1.2 N.D. N.D. -0.9 +0.0
growth on ricinoleic acid YNB Glucose -0.8 N.D. N.D. -0.7 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. N.D. -1.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 N.D. N.D. -1.5 +0.3
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -1.4 +0.1
growth on ypd YPD +0.4 N.D. N.D. -1.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. N.D. -0.3 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. N.D. +0.1 +0.1
auxotrophy Dropout Complete +0.3 N.D. N.D. -0.9 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. N.D. -0.3 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. N.D. +1.2 -1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. -0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.4 N.D. N.D. -0.2 +0.8
auxotrophy YNB Glucose -0.0 N.D. N.D. -0.2 +0.5
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. -0.6 +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. N.D. +1.0 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. N.D. -0.6 +0.6
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. N.D. +0.5 +0.3
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