Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14894 and 14895 are separated by 214 nucleotides14895 and 14896 are separated by 198 nucleotides14896 and 14897 are separated by 297 nucleotides14897 and 14898 are separated by 1115 nucleotides mRNA_6526: 14894 - KOG2929 Transcription factor, component of CCR4 transcriptional complex, at 1,004,889 to 1,006,380 14894 mRNA_6527: 14895 - K18178 COA5, PET191 cytochrome c oxidase assembly factor 5, at 1,006,595 to 1,007,064 14895 mRNA_6528: 14896 - K01514 PRUNE, PPX1 exopolyphosphatase, at 1,007,263 to 1,008,621 14896 mRNA_6529: 14897 - K03626 EGD2, NACA nascent polypeptide-associated complex subunit alpha, at 1,008,919 to 1,009,693 14897 mRNA_6530: 14898 - K03809 wrbA NAD(P)H dehydrogenase (quinone), at 1,010,809 to 1,011,597 14898
Group Condition mRNA_6526 mRNA_6527 mRNA_6528 mRNA_6529 mRNA_6530
14894 14895 14896 14897 14898
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -1.9 -0.7 -0.4
growth on oleic acid YNB Glucose N.D. N.D. -1.4 +0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 +0.2 -0.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -1.2 -0.5 +0.8
growth on ypd YPD N.D. N.D. -1.2 +0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -1.5 +0.3 +0.4
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 -0.1 -0.3
auxotrophy Dropout Complete N.D. N.D. -0.9 +0.6 -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.7 -0.4 +0.6
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.6 +0.8 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.9 +0.0 +0.6
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.6 +0.6 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 +0.1 -0.0
auxotrophy YNB Glucose N.D. N.D. -0.2 +0.5 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.3 -0.0 +0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +1.2 -1.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.1 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.5 +0.3 -0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 +0.8 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +1.0 -0.3 +0.9
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