Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14895 and 14896 are separated by 198 nucleotides14896 and 14897 are separated by 297 nucleotides14897 and 14898 are separated by 1115 nucleotides14898 and 14899 are separated by 379 nucleotides mRNA_6527: 14895 - K18178 COA5, PET191 cytochrome c oxidase assembly factor 5, at 1,006,595 to 1,007,064 14895 mRNA_6528: 14896 - K01514 PRUNE, PPX1 exopolyphosphatase, at 1,007,263 to 1,008,621 14896 mRNA_6529: 14897 - K03626 EGD2, NACA nascent polypeptide-associated complex subunit alpha, at 1,008,919 to 1,009,693 14897 mRNA_6530: 14898 - K03809 wrbA NAD(P)H dehydrogenase (quinone), at 1,010,809 to 1,011,597 14898 mRNA_6531: 14899 - K19940 SEC2 Rab guanine nucleotide exchange factor SEC2, at 1,011,977 to 1,014,833 14899
Group Condition mRNA_6527 mRNA_6528 mRNA_6529 mRNA_6530 mRNA_6531
14895 14896 14897 14898 14899
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.9 -0.7 -0.4 -1.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.2 -0.5 +0.8 -1.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.5 +0.3 +0.4 -1.2
growth on oleic acid YNB Glucose N.D. -1.4 +0.1 -0.3 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 +0.2 -0.8 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.9 +0.0 +0.6 -0.7
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 -0.1 -0.3 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.6 +0.8 -0.4 -0.4
growth on ypd YPD N.D. -1.2 +0.1 +0.3 +0.1
growth on ricinoleic acid YNB Glucose N.D. -0.7 -0.4 +0.6 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +1.2 -1.0 +0.1 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 +0.1 -0.0 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 -0.0 +0.4 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.1 +0.3 -0.4
auxotrophy Dropout Complete N.D. -0.9 +0.6 -0.2 +0.6
auxotrophy YNB Glucose 75mM Arginine N.D. -0.6 +0.6 -0.1 +0.2
auxotrophy YNB Glucose N.D. -0.2 +0.5 -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 +0.1 -0.1 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.2 +0.8 -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.5 +0.3 -0.3 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +1.0 -0.3 +0.9 +0.3
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