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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
14895 and 14896 are separated by 198 nucleotides
14896 and 14897 are separated by 297 nucleotides
14897 and 14898 are separated by 1115 nucleotides
14898 and 14899 are separated by 379 nucleotides
mRNA_6527: 14895 - K18178 COA5, PET191 cytochrome c oxidase assembly factor 5, at 1,006,595 to 1,007,064
14895
mRNA_6528: 14896 - K01514 PRUNE, PPX1 exopolyphosphatase, at 1,007,263 to 1,008,621
14896
mRNA_6529: 14897 - K03626 EGD2, NACA nascent polypeptide-associated complex subunit alpha, at 1,008,919 to 1,009,693
14897
mRNA_6530: 14898 - K03809 wrbA NAD(P)H dehydrogenase (quinone), at 1,010,809 to 1,011,597
14898
mRNA_6531: 14899 - K19940 SEC2 Rab guanine nucleotide exchange factor SEC2, at 1,011,977 to 1,014,833
14899
Group
Condition
mRNA
_6527
mRNA
_6528
mRNA
_6529
mRNA
_6530
mRNA
_6531
14895
14896
14897
14898
14899
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
-1.9
-0.7
-0.4
-1.5
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
-1.2
-0.5
+0.8
-1.5
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
-1.5
+0.3
+0.4
-1.2
growth on oleic acid
YNB Glucose
N.D.
-1.4
+0.1
-0.3
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
-0.3
+0.2
-0.8
-0.4
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
-0.9
+0.0
+0.6
-0.7
ypd buoyancy
40 hrs High Buoyancy
N.D.
-0.3
-0.1
-0.3
-0.3
auxotrophy
YNB Glucose 75mM Methionine
N.D.
-0.6
+0.8
-0.4
-0.4
growth on ypd
YPD
N.D.
-1.2
+0.1
+0.3
+0.1
growth on ricinoleic acid
YNB Glucose
N.D.
-0.7
-0.4
+0.6
-0.0
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
+1.2
-1.0
+0.1
-0.6
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
-0.1
+0.1
-0.0
-0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
-0.3
-0.0
+0.4
-0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
+0.1
+0.1
+0.3
-0.4
auxotrophy
Dropout Complete
N.D.
-0.9
+0.6
-0.2
+0.6
auxotrophy
YNB Glucose 75mM Arginine
N.D.
-0.6
+0.6
-0.1
+0.2
auxotrophy
YNB Glucose
N.D.
-0.2
+0.5
-0.2
+0.1
ypd buoyancy
40 hrs Median Buoyancy
N.D.
+0.2
+0.1
-0.1
+0.0
growth on oleic acid
YNB Oleic Acid
N.D.
-0.2
+0.8
-0.1
-0.2
ypd buoyancy
40 hrs Low Buoyancy
N.D.
+0.5
+0.3
-0.3
+0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
+1.0
-0.3
+0.9
+0.3
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