Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14898 and 14899 are separated by 379 nucleotides14899 and 14900 are separated by 574 nucleotides14900 and 14901 are separated by 522 nucleotides14901 and 14902 are separated by 685 nucleotides mRNA_6530: 14898 - K03809 wrbA NAD(P)H dehydrogenase (quinone), at 1,010,809 to 1,011,597 14898 mRNA_6531: 14899 - K19940 SEC2 Rab guanine nucleotide exchange factor SEC2, at 1,011,977 to 1,014,833 14899 mRNA_6532: 14900 - K11352 NDUFA12 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 12, at 1,015,408 to 1,016,084 14900 mRNA_6533: 14901 - Hypothetical Protein, at 1,016,607 to 1,018,087 14901 mRNA_6534: 14902 - HMMPfam-Opy2 protein-PF09463, at 1,018,773 to 1,021,328 14902
Group Condition mRNA_6530 mRNA_6531 mRNA_6532 mRNA_6533 mRNA_6534
14898 14899 14900 14901 14902
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -1.2 -1.7 +0.8 -3.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 -0.7 -2.2 +0.5 -2.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.6 -0.4 -0.7 -1.0
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -1.5 +0.0 -0.2 -0.6
facs on lipid accumulation Sorted No BODIPY Gate +0.8 -1.5 +0.4 -0.5 -0.9
growth on oleic acid YNB Oleic Acid -0.1 -0.2 -1.2 +0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.4 +0.3 +0.2 -0.6
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.2 +0.1 -0.6 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 -0.4 -1.1 +0.4 +1.1
auxotrophy YNB Glucose -0.2 +0.1 -0.0 -0.0 -0.3
growth on ricinoleic acid YNB Glucose +0.6 -0.0 -0.3 -0.1 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.4 +0.4 +0.0 +0.1
growth on oleic acid YNB Glucose -0.3 +0.0 -0.1 +0.3 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.2 -0.4 +0.7 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.3 +0.3 +1.2 -0.2
auxotrophy Dropout Complete -0.2 +0.6 -0.2 -0.2 +0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.4 +0.6 +0.8 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 +0.5 +0.4 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.4 +1.0 +1.6 -0.9
growth on ypd YPD +0.3 +0.1 +0.4 +2.2 -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.9 +0.3 +1.3 +0.3 -0.7
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