Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14912 and 14913 are separated by 1004 nucleotides14913 and 14914 are separated by 420 nucleotides14914 and 14915 are separated by 164 nucleotides14915 and 14916 are separated by 800 nucleotides mRNA_6544: 14912 - K02138 ATPeF0D, ATP5H, ATP7 F-type H+-transporting ATPase subunit d, at 1,046,957 to 1,048,093 14912 mRNA_6545: 14913 - SUPERFAMILY--SSF103657, at 1,049,098 to 1,050,167 14913 mRNA_6546: 14914 - K01702 LEU1 3-isopropylmalate dehydratase, at 1,050,588 to 1,053,356 14914 mRNA_6547: 14915 - KOG3040 Predicted sugar phosphatase (HAD superfamily), at 1,053,521 to 1,054,436 14915 mRNA_6548: 14916 - K11592 DICER1, DCR1 endoribonuclease Dicer, at 1,055,237 to 1,061,964 14916
Group Condition mRNA_6544 mRNA_6545 mRNA_6546 mRNA_6547 mRNA_6548
14912 14913 14914 14915 14916
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 N.D. N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.4 N.D. N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 N.D. N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. N.D. -0.1
growth on ypd YPD N.D. -0.2 N.D. N.D. +0.0
growth on oleic acid YNB Glucose N.D. +0.1 N.D. N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. N.D. +0.1
auxotrophy YNB Glucose N.D. -0.1 N.D. N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. -0.2 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 N.D. N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 N.D. N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 N.D. N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.2 N.D. N.D. -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 N.D. N.D. +0.2
auxotrophy Dropout Complete N.D. +0.3 N.D. N.D. +0.2
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