Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14913 and 14914 are separated by 420 nucleotides14914 and 14915 are separated by 164 nucleotides14915 and 14916 are separated by 800 nucleotides14916 and 14917 are separated by 994 nucleotides mRNA_6545: 14913 - SUPERFAMILY--SSF103657, at 1,049,098 to 1,050,167 14913 mRNA_6546: 14914 - K01702 LEU1 3-isopropylmalate dehydratase, at 1,050,588 to 1,053,356 14914 mRNA_6547: 14915 - KOG3040 Predicted sugar phosphatase (HAD superfamily), at 1,053,521 to 1,054,436 14915 mRNA_6548: 14916 - K11592 DICER1, DCR1 endoribonuclease Dicer, at 1,055,237 to 1,061,964 14916 mRNA_6549: 14917 - KOG0605 NDR and related serine/threonine kinases, at 1,062,959 to 1,066,157 14917
Group Condition mRNA_6545 mRNA_6546 mRNA_6547 mRNA_6548 mRNA_6549
14913 14914 14915 14916 14917
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. N.D. -0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. N.D. +0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. N.D. -0.1 -0.2
growth on ypd YPD -0.2 N.D. N.D. +0.0 -0.2
growth on oleic acid YNB Glucose +0.1 N.D. N.D. -0.3 -0.2
auxotrophy YNB Glucose -0.1 N.D. N.D. +0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. N.D. +0.0 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. N.D. -0.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. -0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. +0.1 -0.1
growth on ricinoleic acid YNB Glucose -0.2 N.D. N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. -0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.0 -0.1
growth on oleic acid YNB Oleic Acid +0.2 N.D. N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. N.D. +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. +0.2 -0.2
auxotrophy Dropout Complete +0.3 N.D. N.D. +0.2 -0.2
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