Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14914 and 14915 are separated by 164 nucleotides14915 and 14916 are separated by 800 nucleotides14916 and 14917 are separated by 994 nucleotides14917 and 14918 are separated by 69 nucleotides mRNA_6546: 14914 - K01702 LEU1 3-isopropylmalate dehydratase, at 1,050,588 to 1,053,356 14914 mRNA_6547: 14915 - KOG3040 Predicted sugar phosphatase (HAD superfamily), at 1,053,521 to 1,054,436 14915 mRNA_6548: 14916 - K11592 DICER1, DCR1 endoribonuclease Dicer, at 1,055,237 to 1,061,964 14916 mRNA_6549: 14917 - KOG0605 NDR and related serine/threonine kinases, at 1,062,959 to 1,066,157 14917 mRNA_6550: 14918 - K11374 ELP2 elongator complex protein 2, at 1,066,227 to 1,069,568 14918
Group Condition mRNA_6546 mRNA_6547 mRNA_6548 mRNA_6549 mRNA_6550
14914 14915 14916 14917 14918
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 -0.1 -2.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 -0.2 -1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 -0.1 -1.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 -0.0 -1.0
growth on ypd YPD N.D. N.D. +0.0 -0.2 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.0 -0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 -0.1 -0.2
auxotrophy YNB Glucose N.D. N.D. +0.1 -0.2 +0.0
growth on oleic acid YNB Glucose N.D. N.D. -0.3 -0.2 +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.2 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.0 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.0 +0.0 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.0 -0.3 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 -0.2 +0.2
auxotrophy Dropout Complete N.D. N.D. +0.2 -0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 -0.0 +0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 -0.1 +0.4
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.0 -0.1 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.1 -0.3 +0.7
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 -0.3 +0.8
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.0 -0.2 +1.0
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