Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14946 and 14947 are separated by 60 nucleotides14947 and 14948 are separated by 134 nucleotides14948 and 14949 are separated by 392 nucleotides14949 and 14950 are separated by 396 nucleotides mRNA_6578: 14946 - HMMPfam-pfkB family carbohydrate kinase-PF00294,SUPERFAMILY--SSF53613, at 1,147,858 to 1,149,094 14946 mRNA_6579: 14947 - KOG0871 Class 2 transcription repressor NC2, beta subunit (Dr1), at 1,149,155 to 1,149,894 14947 mRNA_6580: 14948 - K11098 SNRPF, SMF small nuclear ribonucleoprotein F, at 1,150,029 to 1,150,564 14948 mRNA_6581: 14949 - HMMPfam-Major Facilitator Superfamily-PF07690,ProSiteProfiles-Major facilitator superfamily (MFS) profile.-PS50850,SUPERFAMILY--SSF103473, at 1,150,957 to 1,153,150 14949 mRNA_6582: 14950 - K11517 HAO (S)-2-hydroxy-acid oxidase, at 1,153,547 to 1,155,730 14950
Group Condition mRNA_6578 mRNA_6579 mRNA_6580 mRNA_6581 mRNA_6582
14946 14947 14948 14949 14950
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. N.D. -0.1 -1.5
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. -0.5 -0.5
auxotrophy Dropout Complete -0.8 N.D. N.D. -0.7 +0.8
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. N.D. -0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. +0.4 -0.4
growth on oleic acid YNB Glucose -0.3 N.D. N.D. +0.6 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. N.D. +0.1 -0.3
growth on ypd YPD +0.4 N.D. N.D. -0.3 -0.1
growth on oleic acid YNB Oleic Acid -0.4 N.D. N.D. +0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.5 N.D. N.D. +0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. N.D. +0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. N.D. +0.2 +0.1
auxotrophy YNB Glucose -0.5 N.D. N.D. -0.0 +0.8
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. N.D. -0.3 +1.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. N.D. +0.5 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. N.D. +0.6 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. N.D. +0.3 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. N.D. +0.1 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. -0.1 +0.8
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. +0.4 +0.3
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