Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14954 and 14955 are separated by 1370 nucleotides14955 and 14956 are separated by 747 nucleotides14956 and 14957 are separated by 441 nucleotides14957 and 14958 are separated by 456 nucleotides mRNA_6586: 14954 - SUPERFAMILY--SSF55729, at 1,163,185 to 1,164,579 14954 mRNA_6587: 14955 - SUPERFAMILY--SSF57184, at 1,165,950 to 1,167,130 14955 mRNA_6588: 14956 - K00966 GMPP mannose-1-phosphate guanylyltransferase, at 1,167,878 to 1,169,516 14956 mRNA_6589: 14957 - K12386 CTNS cystinosin, at 1,169,958 to 1,171,396 14957 mRNA_6590: 14958 - K03009 RPB12, POLR2K DNA-directed RNA polymerases I, II, and III subunit RPABC4, at 1,171,853 to 1,172,203 14958
Group Condition mRNA_6586 mRNA_6587 mRNA_6588 mRNA_6589 mRNA_6590
14954 14955 14956 14957 14958
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.2 N.D. -0.2 -1.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.5 N.D. -0.5 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.2 N.D. -0.1 +0.1
auxotrophy Dropout Complete +0.2 -0.2 N.D. +0.3 -0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.7 N.D. -0.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.1 N.D. -0.2 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.3 N.D. +0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.0 N.D. -0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 -0.7 N.D. -0.6 +0.7
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.4 N.D. -0.2 +0.7
growth on oleic acid YNB Glucose -0.2 +0.1 N.D. -0.0 +0.4
growth on ypd YPD -0.2 -0.3 N.D. -0.0 +0.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 +0.3 N.D. +0.0 -0.5
auxotrophy YNB Glucose +0.3 -0.2 N.D. +0.1 +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.2 N.D. -0.1 +0.7
growth on oleic acid YNB Oleic Acid +0.0 -0.0 N.D. -0.2 +0.8
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.3 N.D. -0.0 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.2 N.D. -0.2 +0.8
growth on ricinoleic acid YNB Glucose +0.6 -0.1 N.D. +0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 +0.3 N.D. +0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.1 N.D. +0.2 +0.5
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