Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14956 and 14957 are separated by 441 nucleotides14957 and 14958 are separated by 456 nucleotides14958 and 14959 are separated by 604 nucleotides14959 and 14960 are separated by 356 nucleotides mRNA_6588: 14956 - K00966 GMPP mannose-1-phosphate guanylyltransferase, at 1,167,878 to 1,169,516 14956 mRNA_6589: 14957 - K12386 CTNS cystinosin, at 1,169,958 to 1,171,396 14957 mRNA_6590: 14958 - K03009 RPB12, POLR2K DNA-directed RNA polymerases I, II, and III subunit RPABC4, at 1,171,853 to 1,172,203 14958 mRNA_6591: 14959 - K05724 FGD5_6 FYVE, RhoGEF and PH domain containing 5/6, at 1,172,808 to 1,176,820 14959 mRNA_6592: 14960 - KOG2571 Chitin synthase/hyaluronan synthase (glycosyltransferases), at 1,177,177 to 1,181,389 14960
Group Condition mRNA_6588 mRNA_6589 mRNA_6590 mRNA_6591 mRNA_6592
14956 14957 14958 14959 14960
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 -1.3 +0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 -0.5 +0.2 -1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.5 -0.2 +0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.6 +0.7 -0.1 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 -0.3 +0.1 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 +0.3 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 +0.2 -0.0 -0.2
auxotrophy Dropout Complete N.D. +0.3 -0.8 +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 +0.3 -0.1 -0.2
growth on oleic acid YNB Glucose N.D. -0.0 +0.4 +0.1 -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.1 -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 -0.2 +0.4 -0.5
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.5 +0.0 -0.6
auxotrophy YNB Glucose N.D. +0.1 +0.4 +0.1 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 +0.6 -0.1 -0.0
growth on ricinoleic acid YNB Glucose N.D. +0.2 +0.2 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 +0.8 +0.0 -0.1
growth on ypd YPD N.D. -0.0 +0.9 -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 +0.7 +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.7 +0.2 +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.2 +0.8 +0.4 +0.4
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