Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14969 and 14970 are separated by 468 nucleotides14970 and 14971 are separated by 416 nucleotides14971 and 14972 are separated by 214 nucleotides14972 and 14973 are separated by 357 nucleotides mRNA_6601: 14969 - K04512 DAAM dishevelled associated activator of morphogenesis, at 1,200,384 to 1,206,346 14969 mRNA_6602: 14970 - K07953 SAR1 GTP-binding protein SAR1, at 1,206,815 to 1,208,411 14970 mRNA_6603: 14971 - K02149 ATPeV1D, ATP6M V-type H+-transporting ATPase subunit D, at 1,208,828 to 1,210,295 14971 mRNA_6604: 14972 - K12812 UAP56, BAT1, SUB2 ATP-dependent RNA helicase UAP56/SUB2, at 1,210,510 to 1,213,080 14972 mRNA_6605: 14973 - KOG2177 Predicted E3 ubiquitin ligase, at 1,213,438 to 1,216,130 14973
Group Condition mRNA_6601 mRNA_6602 mRNA_6603 mRNA_6604 mRNA_6605
14969 14970 14971 14972 14973
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. N.D. N.D. -0.6
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. N.D. N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 N.D. N.D. N.D. +0.2
auxotrophy YNB Glucose -0.3 N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. N.D. N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. N.D. N.D. -0.3
growth on oleic acid YNB Glucose -0.1 N.D. N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.1 N.D. N.D. N.D. -0.1
growth on ypd YPD -0.1 N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. N.D. N.D. +0.1
auxotrophy Dropout Complete +0.1 N.D. N.D. N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. N.D. N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.6 N.D. N.D. N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.6 N.D. N.D. N.D. +0.1
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