Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14971 and 14972 are separated by 214 nucleotides14972 and 14973 are separated by 357 nucleotides14973 and 14974 are separated by 91 nucleotides14974 and 14975 are separated by 348 nucleotides mRNA_6603: 14971 - K02149 ATPeV1D, ATP6M V-type H+-transporting ATPase subunit D, at 1,208,828 to 1,210,295 14971 mRNA_6604: 14972 - K12812 UAP56, BAT1, SUB2 ATP-dependent RNA helicase UAP56/SUB2, at 1,210,510 to 1,213,080 14972 mRNA_6605: 14973 - KOG2177 Predicted E3 ubiquitin ligase, at 1,213,438 to 1,216,130 14973 mRNA_6606: 14974 - KOG2761 START domain-containing proteins involved in steroidogenesis/phosphatidylcholine transfer, at 1,216,222 to 1,217,950 14974 mRNA_6607: 14975 - K14572 MDN1, REA1 midasin, at 1,218,299 to 1,235,391 14975
Group Condition mRNA_6603 mRNA_6604 mRNA_6605 mRNA_6606 mRNA_6607
14971 14972 14973 14974 14975
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 -0.7 N.D.
growth on oleic acid YNB Glucose N.D. N.D. -0.2 -0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.6 -0.1 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 -0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.0 -0.6 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.3 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.2 -0.6 N.D.
auxotrophy YNB Glucose N.D. N.D. -0.1 -0.2 N.D.
growth on ypd YPD N.D. N.D. -0.1 -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 +0.1 N.D.
auxotrophy Dropout Complete N.D. N.D. -0.2 +0.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.0 +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.3 +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.1 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.1 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.0 +0.5 N.D.
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