Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14974 and 14975 are separated by 348 nucleotides14975 and 14976 are separated by 176 nucleotides14976 and 14977 are separated by 621 nucleotides14977 and 14978 are separated by 194 nucleotides mRNA_6606: 14974 - KOG2761 START domain-containing proteins involved in steroidogenesis/phosphatidylcholine transfer, at 1,216,222 to 1,217,950 14974 mRNA_6607: 14975 - K14572 MDN1, REA1 midasin, at 1,218,299 to 1,235,391 14975 mRNA_6608: 14976 - HMMPfam-Yqey-like protein-PF09424,SUPERFAMILY--SSF89095, at 1,235,568 to 1,236,163 14976 mRNA_6609: 14977 - KOG1232 Proteins containing the FAD binding domain, at 1,236,785 to 1,239,365 14977 mRNA_6610: 14978 - BLAST related to MSL5-branch point bridging protein [Sporis..., at 1,239,560 to 1,240,604 14978
Group Condition mRNA_6606 mRNA_6607 mRNA_6608 mRNA_6609 mRNA_6610
14974 14975 14976 14977 14978
growth on ypd YPD -0.1 N.D. -0.2 -0.8 -1.8
facs on lipid accumulation Sorted High BODIPY Gate -0.7 N.D. -1.0 -0.5 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.4 -0.2 -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.6 -0.1 +0.1
growth on ricinoleic acid YNB Glucose -0.6 N.D. -0.1 +0.4 -0.1
auxotrophy YNB Glucose -0.2 N.D. +0.0 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.7 N.D. +0.0 +0.0 +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.1 +0.2 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. -0.4 +0.4 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. -0.1 +0.0 +0.6
auxotrophy Dropout Complete +0.1 N.D. -0.3 +0.0 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 N.D. +0.3 -0.2 +0.8
facs on lipid accumulation Sorted No BODIPY Gate +0.4 N.D. -0.4 -0.0 +0.3
growth on oleic acid YNB Glucose -0.6 N.D. -0.1 +0.0 +0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 N.D. +0.5 -0.2 +0.7
growth on oleic acid YNB Oleic Acid +0.0 N.D. -0.2 -0.1 +0.8
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.6 +0.5 -0.8
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.0 -0.1 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. -0.0 -0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +1.0 +0.2 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.3 +0.5 +0.1
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