Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15003 and 15004 are separated by 187 nucleotides15004 and 15005 are separated by 210 nucleotides15005 and 15006 are separated by 223 nucleotides15006 and 15007 are separated by 1116 nucleotides mRNA_6635: 15003 - HMMPfam-Peptidase family M48-PF01435, at 1,302,314 to 1,305,791 15003 mRNA_6636: 15004 - K14403 CPSF3, YSH1 cleavage and polyadenylation specificity factor subunit 3, at 1,305,979 to 1,309,273 15004 mRNA_6637: 15005 - K15711 SMARCA3, HLTF SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A3, at 1,309,484 to 1,313,910 15005 mRNA_6638: 15006 - K02575 NRT, narK, nrtP, nasA MFS transporter, NNP family, nitrate/nitrite transporter, at 1,314,134 to 1,316,580 15006 mRNA_6639: 15007 - K00362 nirB nitrite reductase (NADH) large subunit, at 1,317,697 to 1,321,815 15007
Group Condition mRNA_6635 mRNA_6636 mRNA_6637 mRNA_6638 mRNA_6639
15003 15004 15005 15006 15007
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. N.D. -1.0 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -0.9 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. -0.5 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. N.D. -0.1 -0.3
growth on oleic acid YNB Glucose +0.2 N.D. N.D. -0.3 +0.0
auxotrophy Dropout Complete +0.2 N.D. N.D. -0.8 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. +0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.0 +0.0
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. -0.0 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. N.D. +0.1 +0.1
auxotrophy YNB Glucose +0.3 N.D. N.D. -0.3 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. N.D. -0.3 +0.3
growth on ricinoleic acid YNB Glucose +0.2 N.D. N.D. -0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. N.D. -0.3 +0.2
growth on ypd YPD +0.1 N.D. N.D. +0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. N.D. -0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. N.D. -0.0 +0.2
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