Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15004 and 15005 are separated by 210 nucleotides15005 and 15006 are separated by 223 nucleotides15006 and 15007 are separated by 1116 nucleotides15007 and 15008 are separated by 671 nucleotides mRNA_6636: 15004 - K14403 CPSF3, YSH1 cleavage and polyadenylation specificity factor subunit 3, at 1,305,979 to 1,309,273 15004 mRNA_6637: 15005 - K15711 SMARCA3, HLTF SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A3, at 1,309,484 to 1,313,910 15005 mRNA_6638: 15006 - K02575 NRT, narK, nrtP, nasA MFS transporter, NNP family, nitrate/nitrite transporter, at 1,314,134 to 1,316,580 15006 mRNA_6639: 15007 - K00362 nirB nitrite reductase (NADH) large subunit, at 1,317,697 to 1,321,815 15007 mRNA_6640: 15008 - K10534 NR nitrate reductase (NAD(P)H), at 1,322,487 to 1,325,707 15008
Group Condition mRNA_6636 mRNA_6637 mRNA_6638 mRNA_6639 mRNA_6640
15004 15005 15006 15007 15008
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.0 -0.3 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.5 -0.0 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.9 +0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.1 -0.3 -0.0
growth on oleic acid YNB Glucose N.D. N.D. -0.3 +0.0 -0.0
auxotrophy Dropout Complete N.D. N.D. -0.8 +0.4 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.2 -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 +0.0 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.3 +0.2 +0.1
auxotrophy YNB Glucose N.D. N.D. -0.3 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.0 -0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.0 +0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 +0.2 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.2 -0.1 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.0 +0.2 +0.0
growth on ypd YPD N.D. N.D. +0.3 -0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 +0.3 +0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.0 +0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.1 +0.1 +0.2
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