Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15005 and 15006 are separated by 223 nucleotides15006 and 15007 are separated by 1116 nucleotides15007 and 15008 are separated by 671 nucleotides15008 and 15009 are separated by 651 nucleotides mRNA_6637: 15005 - K15711 SMARCA3, HLTF SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A3, at 1,309,484 to 1,313,910 15005 mRNA_6638: 15006 - K02575 NRT, narK, nrtP, nasA MFS transporter, NNP family, nitrate/nitrite transporter, at 1,314,134 to 1,316,580 15006 mRNA_6639: 15007 - K00362 nirB nitrite reductase (NADH) large subunit, at 1,317,697 to 1,321,815 15007 mRNA_6640: 15008 - K10534 NR nitrate reductase (NAD(P)H), at 1,322,487 to 1,325,707 15008 mRNA_6641: 15009 - K11863 ATXN3, MJD Ataxin-3, at 1,326,359 to 1,327,984 15009
Group Condition mRNA_6637 mRNA_6638 mRNA_6639 mRNA_6640 mRNA_6641
15005 15006 15007 15008 15009
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.0 -0.3 -0.4 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.9 +0.1 +0.3 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 -0.0 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 -0.2 -0.1 -0.4
auxotrophy Dropout Complete N.D. -0.8 +0.4 +0.2 -0.1
growth on oleic acid YNB Glucose N.D. -0.3 +0.0 -0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 +0.2 +0.1 -0.2
growth on ypd YPD N.D. +0.3 -0.1 -0.0 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 +0.2 +0.0 -0.3
auxotrophy YNB Glucose N.D. -0.3 +0.2 +0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 +0.1 +0.2 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 -0.1 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.2 -0.1 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 +0.3 +0.3 -0.3
growth on ricinoleic acid YNB Glucose N.D. -0.0 +0.1 +0.3 -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.0 +0.0 +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 -0.0 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -0.1 +0.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 -0.3 -0.0 +0.8
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.0 -0.0 +0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 +0.2 +0.0 +0.8
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