Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15006 and 15007 are separated by 1116 nucleotides15007 and 15008 are separated by 671 nucleotides15008 and 15009 are separated by 651 nucleotides15009 and 15010 are separated by 199 nucleotides mRNA_6638: 15006 - K02575 NRT, narK, nrtP, nasA MFS transporter, NNP family, nitrate/nitrite transporter, at 1,314,134 to 1,316,580 15006 mRNA_6639: 15007 - K00362 nirB nitrite reductase (NADH) large subunit, at 1,317,697 to 1,321,815 15007 mRNA_6640: 15008 - K10534 NR nitrate reductase (NAD(P)H), at 1,322,487 to 1,325,707 15008 mRNA_6641: 15009 - K11863 ATXN3, MJD Ataxin-3, at 1,326,359 to 1,327,984 15009 mRNA_6642: 15010 - KOG2684 Sirtuin 5 and related class III sirtuins (SIR2 family), at 1,328,184 to 1,330,070 15010
Group Condition mRNA_6638 mRNA_6639 mRNA_6640 mRNA_6641 mRNA_6642
15006 15007 15008 15009 15010
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.3 -0.4 -0.5 -0.6
auxotrophy YNB Glucose -0.3 +0.2 +0.1 -0.2 -0.7
auxotrophy Dropout Complete -0.8 +0.4 +0.2 -0.1 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.9 +0.1 +0.3 -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.5 -0.0 +0.0 +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.2 +0.0 -0.3 -0.4
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.2 +0.1 -0.2 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.2 -0.1 -0.4 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.2 -0.1 +0.0 -0.3
growth on oleic acid YNB Glucose -0.3 +0.0 -0.0 +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.3 +0.3 -0.3 -0.2
growth on ypd YPD +0.3 -0.1 -0.0 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.1 +0.1 -0.1 -0.0
growth on ricinoleic acid YNB Glucose -0.0 +0.1 +0.3 -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.1 +0.2 -0.5 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 +0.1 +0.3 -0.0
growth on oleic acid YNB Oleic Acid -0.0 +0.0 +0.1 +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.0 -0.0 +0.4 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.1 +0.0 +0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.3 -0.0 +0.8 +0.5
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.2 +0.0 +0.8 +0.4
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