Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15008 and 15009 are separated by 651 nucleotides15009 and 15010 are separated by 199 nucleotides15010 and 15011 are separated by 204 nucleotides15011 and 15012 are separated by 408 nucleotides mRNA_6640: 15008 - K10534 NR nitrate reductase (NAD(P)H), at 1,322,487 to 1,325,707 15008 mRNA_6641: 15009 - K11863 ATXN3, MJD Ataxin-3, at 1,326,359 to 1,327,984 15009 mRNA_6642: 15010 - KOG2684 Sirtuin 5 and related class III sirtuins (SIR2 family), at 1,328,184 to 1,330,070 15010 mRNA_6643: 15011 - KOG2983 Uncharacterized conserved protein, at 1,330,275 to 1,331,625 15011 mRNA_6644: 15012 - HMMPfam-Permease for cytosine/purines, uracil, thiamine, allantoin-PF02133,PIRSF--PIRSF002744, at 1,332,034 to 1,334,144 15012
Group Condition mRNA_6640 mRNA_6641 mRNA_6642 mRNA_6643 mRNA_6644
15008 15009 15010 15011 15012
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.5 -0.6 N.D. -0.5
auxotrophy YNB Glucose +0.1 -0.2 -0.7 N.D. -0.6
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.3 -0.4 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 -0.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.4 -0.0 N.D. -0.1
auxotrophy Dropout Complete +0.2 -0.1 -0.4 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.5 +0.1 N.D. -0.4
growth on ypd YPD -0.0 -0.4 +0.1 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.3 -0.3 -0.1 N.D. -0.3
growth on oleic acid YNB Glucose -0.0 +0.1 -0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.0 -0.3 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.1 -0.1 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.3 -0.2 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.2 +0.1 N.D. -0.2
growth on oleic acid YNB Oleic Acid +0.1 +0.1 +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 -0.0 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.4 +0.0 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.3 -0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.2 +0.1 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.8 +0.4 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.8 +0.5 N.D. +0.3
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