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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
15011 and 15012 are separated by 408 nucleotides
15012 and 15013 are separated by 1409 nucleotides
15013 and 15014 are separated by 358 nucleotides
15014 and 15015 are separated by 259 nucleotides
mRNA_6643: 15011 - KOG2983 Uncharacterized conserved protein, at 1,330,275 to 1,331,625
15011
mRNA_6644: 15012 - HMMPfam-Permease for cytosine/purines, uracil, thiamine, allantoin-PF02133,PIRSF--PIRSF002744, at 1,332,034 to 1,334,144
15012
mRNA_6645: 15013 - HMMPfam-GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p-PF13839, at 1,335,554 to 1,337,313
15013
mRNA_6646: 15014 - K14647 vpr minor extracellular serine protease Vpr, at 1,337,672 to 1,341,391
15014
mRNA_6647: 15015 - HMMPfam-Peroxisomal biogenesis factor 11 (PEX11)-PF05648, at 1,341,651 to 1,342,937
15015
Group
Condition
mRNA
_6643
mRNA
_6644
mRNA
_6645
mRNA
_6646
mRNA
_6647
15011
15012
15013
15014
15015
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
-0.2
-0.2
-0.1
-0.9
growth on oleic acid
YNB Glucose
N.D.
-0.3
-0.2
-0.3
-0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
-0.1
-0.0
-0.2
-0.6
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
+0.0
-0.1
-0.2
-0.5
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
-0.5
-0.0
+0.0
-0.2
auxotrophy
YNB Glucose
N.D.
-0.6
-0.3
-0.1
+0.2
growth on ricinoleic acid
YNB Glucose
N.D.
-0.3
-0.0
-0.2
-0.1
growth on ypd
YPD
N.D.
-0.1
-0.3
+0.1
-0.3
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
-0.4
-0.0
-0.0
-0.1
growth on oleic acid
YNB Oleic Acid
N.D.
-0.3
-0.1
-0.2
+0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
-0.1
+0.2
+0.1
-0.5
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
-0.3
+0.2
-0.2
+0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
+0.2
+0.2
-0.4
-0.4
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
+0.3
+0.1
-0.2
-0.5
auxotrophy
Dropout Complete
N.D.
-0.2
-0.1
-0.2
+0.4
auxotrophy
YNB Glucose 75mM Arginine
N.D.
-0.2
-0.1
-0.2
+0.4
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
+0.3
-0.0
+0.0
-0.3
ypd buoyancy
40 hrs Median Buoyancy
N.D.
+0.1
+0.0
-0.1
+0.1
auxotrophy
YNB Glucose 75mM Methionine
N.D.
-0.2
-0.1
+0.1
+0.4
ypd buoyancy
40 hrs High Buoyancy
N.D.
+0.1
+0.3
-0.2
+0.1
ypd buoyancy
40 hrs Low Buoyancy
N.D.
+0.1
+0.4
+0.0
+0.1
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