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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
15014 and 15015 are separated by 259 nucleotides
15015 and 15016 are separated by 60 nucleotides
15016 and 15017 are separated by 364 nucleotides
15017 and 15018 are separated by 612 nucleotides
mRNA_6646: 15014 - K14647 vpr minor extracellular serine protease Vpr, at 1,337,672 to 1,341,391
15014
mRNA_6647: 15015 - HMMPfam-Peroxisomal biogenesis factor 11 (PEX11)-PF05648, at 1,341,651 to 1,342,937
15015
mRNA_6648: 15016 - BLAST Gluconolactonase [Termitomyces sp. J132], at 1,342,998 to 1,344,316
15016
mRNA_6649: 15017 - K12580 CNOT3, NOT3 CCR4-NOT transcription complex subunit 3, at 1,344,681 to 1,347,468
15017
mRNA_6650: 15018 - HMMPfam-Domain of unknown function (DUF1772)-PF08592, at 1,348,081 to 1,348,867
15018
Group
Condition
mRNA
_6646
mRNA
_6647
mRNA
_6648
mRNA
_6649
mRNA
_6650
15014
15015
15016
15017
15018
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.0
-0.2
-0.7
-3.2
+0.3
facs on lipid accumulation
Sorted No BODIPY Gate
-0.2
+0.0
-0.1
-3.6
+0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
-0.9
-0.0
-1.9
-0.2
growth on ricinoleic acid
YNB Glucose
-0.2
-0.1
+0.1
-2.7
+0.1
facs on lipid accumulation
Sorted High BODIPY Gate
-0.0
-0.1
-0.1
-1.9
-0.0
auxotrophy
YNB Glucose
-0.1
+0.2
+0.6
-2.4
+0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
-0.5
+0.3
-1.8
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
-0.6
-0.0
+0.1
+0.2
growth on ypd
YPD
+0.1
-0.3
-0.2
-0.2
+0.2
auxotrophy
YNB Glucose 75mM Arginine
-0.2
+0.4
+0.5
-1.2
+0.1
growth on oleic acid
YNB Oleic Acid
-0.2
+0.0
+0.5
-0.5
+0.0
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.2
-0.5
-0.0
+0.9
+0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.4
-0.4
+0.1
+1.1
-0.1
growth on oleic acid
YNB Glucose
-0.3
-0.1
+0.4
+0.2
+0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.0
+0.1
-0.1
+0.6
-0.3
auxotrophy
Dropout Complete
-0.2
+0.4
+0.4
+0.4
-0.2
ypd buoyancy
40 hrs High Buoyancy
-0.2
+0.1
-0.0
+1.5
-0.5
ypd buoyancy
40 hrs No Buoyancy Separation
+0.0
-0.3
+0.2
+0.8
+0.2
auxotrophy
YNB Glucose 75mM Methionine
+0.1
+0.4
+0.7
+0.1
-0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
-0.5
+0.3
+1.2
+0.4
ypd buoyancy
40 hrs Median Buoyancy
-0.1
+0.1
+0.2
+1.4
-0.1
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