Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15014 and 15015 are separated by 259 nucleotides15015 and 15016 are separated by 60 nucleotides15016 and 15017 are separated by 364 nucleotides15017 and 15018 are separated by 612 nucleotides mRNA_6646: 15014 - K14647 vpr minor extracellular serine protease Vpr, at 1,337,672 to 1,341,391 15014 mRNA_6647: 15015 - HMMPfam-Peroxisomal biogenesis factor 11 (PEX11)-PF05648, at 1,341,651 to 1,342,937 15015 mRNA_6648: 15016 - BLAST Gluconolactonase [Termitomyces sp. J132], at 1,342,998 to 1,344,316 15016 mRNA_6649: 15017 - K12580 CNOT3, NOT3 CCR4-NOT transcription complex subunit 3, at 1,344,681 to 1,347,468 15017 mRNA_6650: 15018 - HMMPfam-Domain of unknown function (DUF1772)-PF08592, at 1,348,081 to 1,348,867 15018
Group Condition mRNA_6646 mRNA_6647 mRNA_6648 mRNA_6649 mRNA_6650
15014 15015 15016 15017 15018
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.2 -0.7 -3.2 +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.0 -0.1 -3.6 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.9 -0.0 -1.9 -0.2
growth on ricinoleic acid YNB Glucose -0.2 -0.1 +0.1 -2.7 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.1 -0.1 -1.9 -0.0
auxotrophy YNB Glucose -0.1 +0.2 +0.6 -2.4 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.5 +0.3 -1.8 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.6 -0.0 +0.1 +0.2
growth on ypd YPD +0.1 -0.3 -0.2 -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.4 +0.5 -1.2 +0.1
growth on oleic acid YNB Oleic Acid -0.2 +0.0 +0.5 -0.5 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.5 -0.0 +0.9 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.4 +0.1 +1.1 -0.1
growth on oleic acid YNB Glucose -0.3 -0.1 +0.4 +0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.1 -0.1 +0.6 -0.3
auxotrophy Dropout Complete -0.2 +0.4 +0.4 +0.4 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.1 -0.0 +1.5 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.3 +0.2 +0.8 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.4 +0.7 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.5 +0.3 +1.2 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 +0.2 +1.4 -0.1
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