Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15017 and 15018 are separated by 612 nucleotides15018 and 15019 are separated by 461 nucleotides15019 and 15020 are separated by 1713 nucleotides15020 and 15021 are separated by 1033 nucleotides mRNA_6649: 15017 - K12580 CNOT3, NOT3 CCR4-NOT transcription complex subunit 3, at 1,344,681 to 1,347,468 15017 mRNA_6650: 15018 - HMMPfam-Domain of unknown function (DUF1772)-PF08592, at 1,348,081 to 1,348,867 15018 mRNA_6651: 15019 - K20793 NAA50, NAT5 N-alpha-acetyltransferase 50, at 1,349,329 to 1,350,495 15019 mRNA_6652: 15020 - K02975 RP-S25e, RPS25 small subunit ribosomal protein S25e, at 1,352,209 to 1,353,144 15020 mRNA_6653: 15021 - K04739 PRKAR cAMP-dependent protein kinase regulator, at 1,354,178 to 1,355,889 15021
Group Condition mRNA_6649 mRNA_6650 mRNA_6651 mRNA_6652 mRNA_6653
15017 15018 15019 15020 15021
facs on lipid accumulation Sorted Low BODIPY Gate -3.2 +0.3 +0.3 -0.9 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -3.6 +0.3 +0.4 -0.5 N.D.
growth on ricinoleic acid YNB Glucose -2.7 +0.1 +0.3 -0.7 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -1.9 -0.0 -0.1 -1.0 N.D.
auxotrophy YNB Glucose -2.4 +0.0 +0.4 -0.7 N.D.
auxotrophy YNB Glucose 75mM Arginine -1.2 +0.1 +0.1 -1.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -1.9 -0.2 -0.0 -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -1.8 +0.1 +0.1 -0.2 N.D.
auxotrophy Dropout Complete +0.4 -0.2 -0.3 -1.6 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.2 -0.3 -1.2 N.D.
growth on oleic acid YNB Oleic Acid -0.5 +0.0 -0.5 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 +0.1 -0.7 N.D.
growth on oleic acid YNB Glucose +0.2 +0.2 -0.1 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +1.5 -0.5 +0.1 -1.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.1 -0.1 -0.0 -0.9 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.6 -0.3 +0.2 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +1.4 -0.1 +0.2 -1.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.2 +0.4 -0.4 -0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 +0.1 +0.2 -0.5 N.D.
growth on ypd YPD -0.2 +0.2 -0.1 +0.9 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 +0.2 +0.1 +0.3 N.D.
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