Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15019 and 15020 are separated by 1713 nucleotides15020 and 15021 are separated by 1033 nucleotides15021 and 15022 are separated by 278 nucleotides15022 and 15023 are separated by 1383 nucleotides mRNA_6651: 15019 - K20793 NAA50, NAT5 N-alpha-acetyltransferase 50, at 1,349,329 to 1,350,495 15019 mRNA_6652: 15020 - K02975 RP-S25e, RPS25 small subunit ribosomal protein S25e, at 1,352,209 to 1,353,144 15020 mRNA_6653: 15021 - K04739 PRKAR cAMP-dependent protein kinase regulator, at 1,354,178 to 1,355,889 15021 mRNA_6654: 15022 - K12462 ARHGDI, RHOGDI Rho GDP-dissociation inhibitor, at 1,356,168 to 1,357,307 15022 mRNA_6655: 15023 - Interpro Domain of unknown function (DUF1772), at 1,358,691 to 1,359,757 15023
Group Condition mRNA_6651 mRNA_6652 mRNA_6653 mRNA_6654 mRNA_6655
15019 15020 15021 15022 15023
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -1.2 N.D. -0.4 +0.2
auxotrophy Dropout Complete -0.3 -1.6 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.9 N.D. -0.8 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -1.0 N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.6 N.D. -0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.9 N.D. -0.4 -0.2
growth on ricinoleic acid YNB Glucose +0.3 -0.7 N.D. -0.6 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 -1.4 N.D. +0.2 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.7 N.D. -0.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.4 N.D. -0.4 +0.1
auxotrophy YNB Glucose +0.4 -0.7 N.D. -0.4 +0.1
growth on oleic acid YNB Oleic Acid -0.5 -0.2 N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.5 N.D. -0.5 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.2 -1.1 N.D. +0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 -1.0 N.D. +0.4 +0.2
growth on oleic acid YNB Glucose -0.1 -0.4 N.D. +0.3 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.5 N.D. +0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.2 N.D. +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.2 N.D. +0.4 +0.0
growth on ypd YPD -0.1 +0.9 N.D. -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.3 N.D. +0.4 -0.0
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