Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15029 and 15030 are separated by 1441 nucleotides15030 and 15031 are separated by 456 nucleotides15031 and 15032 are separated by 186 nucleotides15032 and 15033 are separated by 1250 nucleotides mRNA_6661: 15029 - K11699 RDR, RDRP RNA-dependent RNA polymerase, at 1,375,555 to 1,377,901 15029 mRNA_6662: 15030 - HMMPfam-G-patch domain-PF01585,HMMPfam-RNA recognition motif (a.k.a. RRM, RBD, or RNP domain)-PF14259,ProSiteProfiles-Eukaryotic RNA Recognition Motif (RRM) profile.-PS50102,ProSiteProfiles-Zinc finger C2H2 type domain profile.-PS50157,ProSiteProfiles-G-patch domain profile.-PS50174,SMART-RNA recognition motif-SM00360,SMART-glycine rich nucleic binding domain-SM00443,SUPERFAMILY--SSF54928, at 1,379,343 to 1,382,272 15030 mRNA_6663: 15031 - Hypothetical Protein, at 1,382,729 to 1,383,844 15031 mRNA_6664: 15032 - K03165 TOP3 DNA topoisomerase III, at 1,384,031 to 1,388,134 15032 mRNA_6665: 15033 - K01952 purL, PFAS phosphoribosylformylglycinamidine synthase, at 1,389,385 to 1,393,556 15033
Group Condition mRNA_6661 mRNA_6662 mRNA_6663 mRNA_6664 mRNA_6665
15029 15030 15031 15032 15033
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -2.3 +0.1 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -1.7 -0.3 -0.2 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -1.3 +0.1 -0.3 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.7 -0.4 +0.2 -1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.5 -0.2 -0.3 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.2 -0.2 -0.2 -0.5
growth on ricinoleic acid YNB Glucose +0.1 -0.7 +0.1 -1.0 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.2 +0.3 -0.5 -0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.5 -0.0 +0.2 -0.2
auxotrophy YNB Glucose +0.4 -0.3 +0.0 -1.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -1.3 -0.4 +1.0 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.5 -0.1 -0.3 +0.6
growth on ypd YPD -0.1 -0.2 +0.4 -0.5 +0.3
growth on oleic acid YNB Oleic Acid -0.0 +0.3 +0.1 +0.2 -0.5
growth on oleic acid YNB Glucose -0.0 +0.1 +0.3 -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.2 +0.0 -0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.5 +0.2 +0.2 +0.5
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.5 +0.3 +0.9 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.3 +0.4 +0.0 +0.8
auxotrophy Dropout Complete +0.3 -0.3 +0.3 -0.2 +0.8
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.5 +0.6 +0.8 +0.3
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