Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15031 and 15032 are separated by 186 nucleotides15032 and 15033 are separated by 1250 nucleotides15033 and 15034 are separated by 228 nucleotides15034 and 15035 are separated by 278 nucleotides mRNA_6663: 15031 - Hypothetical Protein, at 1,382,729 to 1,383,844 15031 mRNA_6664: 15032 - K03165 TOP3 DNA topoisomerase III, at 1,384,031 to 1,388,134 15032 mRNA_6665: 15033 - K01952 purL, PFAS phosphoribosylformylglycinamidine synthase, at 1,389,385 to 1,393,556 15033 mRNA_6666: 15034 - K18423 CSE1, CAS, XPO2 exportin-2 (importin alpha re-exporter), at 1,393,785 to 1,397,625 15034 mRNA_6667: 15035 - Hypothetical Protein, at 1,397,904 to 1,402,835 15035
Group Condition mRNA_6663 mRNA_6664 mRNA_6665 mRNA_6666 mRNA_6667
15031 15032 15033 15034 15035
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.2 -1.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.3 -0.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.2 -0.5 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.1 -1.0 +0.1 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.5 -0.6 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.3 -0.5 N.D. N.D.
auxotrophy YNB Glucose +0.0 -1.1 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.2 -0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.1 +0.2 -0.5 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.2 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.2 +0.0 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.1 +0.1 N.D. N.D.
growth on oleic acid YNB Glucose +0.3 -0.1 -0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.3 +0.6 N.D. N.D.
growth on ypd YPD +0.4 -0.5 +0.3 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +1.0 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.2 +0.5 N.D. N.D.
auxotrophy Dropout Complete +0.3 -0.2 +0.8 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.9 +0.0 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.0 +0.8 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.6 +0.8 +0.3 N.D. N.D.
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