Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15032 and 15033 are separated by 1250 nucleotides15033 and 15034 are separated by 228 nucleotides15034 and 15035 are separated by 278 nucleotides15035 and 15036 are separated by 698 nucleotides mRNA_6664: 15032 - K03165 TOP3 DNA topoisomerase III, at 1,384,031 to 1,388,134 15032 mRNA_6665: 15033 - K01952 purL, PFAS phosphoribosylformylglycinamidine synthase, at 1,389,385 to 1,393,556 15033 mRNA_6666: 15034 - K18423 CSE1, CAS, XPO2 exportin-2 (importin alpha re-exporter), at 1,393,785 to 1,397,625 15034 mRNA_6667: 15035 - Hypothetical Protein, at 1,397,904 to 1,402,835 15035 mRNA_6668: 15036 - K03509 POLH DNA polymerase eta, at 1,403,534 to 1,406,837 15036
Group Condition mRNA_6664 mRNA_6665 mRNA_6666 mRNA_6667 mRNA_6668
15032 15033 15034 15035 15036
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.6 N.D. N.D. -0.3
growth on ricinoleic acid YNB Glucose -1.0 +0.1 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.7 N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -1.3 N.D. N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.5 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.5 N.D. N.D. +0.0
auxotrophy YNB Glucose -1.1 +0.3 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.0 N.D. N.D. -0.3
growth on oleic acid YNB Glucose -0.1 -0.1 N.D. N.D. -0.2
growth on oleic acid YNB Oleic Acid +0.2 -0.5 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.0 N.D. N.D. -0.1
growth on ypd YPD -0.5 +0.3 N.D. N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.1 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.6 N.D. N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.2 N.D. N.D. +0.4
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.5 N.D. N.D. -0.1
auxotrophy Dropout Complete -0.2 +0.8 N.D. N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.8 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.9 +0.0 N.D. N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.8 +0.3 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +1.0 +0.2 N.D. N.D. +0.1
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