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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
15032 and 15033 are separated by 1250 nucleotides
15033 and 15034 are separated by 228 nucleotides
15034 and 15035 are separated by 278 nucleotides
15035 and 15036 are separated by 698 nucleotides
mRNA_6664: 15032 - K03165 TOP3 DNA topoisomerase III, at 1,384,031 to 1,388,134
15032
mRNA_6665: 15033 - K01952 purL, PFAS phosphoribosylformylglycinamidine synthase, at 1,389,385 to 1,393,556
15033
mRNA_6666: 15034 - K18423 CSE1, CAS, XPO2 exportin-2 (importin alpha re-exporter), at 1,393,785 to 1,397,625
15034
mRNA_6667: 15035 - Hypothetical Protein, at 1,397,904 to 1,402,835
15035
mRNA_6668: 15036 - K03509 POLH DNA polymerase eta, at 1,403,534 to 1,406,837
15036
Group
Condition
mRNA
_6664
mRNA
_6665
mRNA
_6666
mRNA
_6667
mRNA
_6668
15032
15033
15034
15035
15036
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.5
-0.6
N.D.
N.D.
-0.3
growth on ricinoleic acid
YNB Glucose
-1.0
+0.1
N.D.
N.D.
-0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.3
-0.7
N.D.
N.D.
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
+0.2
-1.3
N.D.
N.D.
-0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.3
-0.5
N.D.
N.D.
-0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.2
-0.5
N.D.
N.D.
+0.0
auxotrophy
YNB Glucose
-1.1
+0.3
N.D.
N.D.
+0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.2
-0.0
N.D.
N.D.
-0.3
growth on oleic acid
YNB Glucose
-0.1
-0.1
N.D.
N.D.
-0.2
growth on oleic acid
YNB Oleic Acid
+0.2
-0.5
N.D.
N.D.
-0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
+0.0
N.D.
N.D.
-0.1
growth on ypd
YPD
-0.5
+0.3
N.D.
N.D.
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.1
+0.1
N.D.
N.D.
+0.1
auxotrophy
YNB Glucose 75mM Methionine
-0.3
+0.6
N.D.
N.D.
+0.0
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.2
-0.2
N.D.
N.D.
+0.4
auxotrophy
YNB Glucose 75mM Arginine
+0.2
+0.5
N.D.
N.D.
-0.1
auxotrophy
Dropout Complete
-0.2
+0.8
N.D.
N.D.
+0.1
ypd buoyancy
40 hrs High Buoyancy
+0.0
+0.8
N.D.
N.D.
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.9
+0.0
N.D.
N.D.
+0.0
ypd buoyancy
40 hrs Low Buoyancy
+0.8
+0.3
N.D.
N.D.
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+1.0
+0.2
N.D.
N.D.
+0.1
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