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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
15033 and 15034 are separated by 228 nucleotides
15034 and 15035 are separated by 278 nucleotides
15035 and 15036 are separated by 698 nucleotides
15036 and 15037 are separated by 168 nucleotides
mRNA_6665: 15033 - K01952 purL, PFAS phosphoribosylformylglycinamidine synthase, at 1,389,385 to 1,393,556
15033
mRNA_6666: 15034 - K18423 CSE1, CAS, XPO2 exportin-2 (importin alpha re-exporter), at 1,393,785 to 1,397,625
15034
mRNA_6667: 15035 - Hypothetical Protein, at 1,397,904 to 1,402,835
15035
mRNA_6668: 15036 - K03509 POLH DNA polymerase eta, at 1,403,534 to 1,406,837
15036
mRNA_6669: 15037 - K03564 BCP, PRXQ, DOT5 peroxiredoxin Q/BCP, at 1,407,006 to 1,408,136
15037
Group
Condition
mRNA
_6665
mRNA
_6666
mRNA
_6667
mRNA
_6668
mRNA
_6669
15033
15034
15035
15036
15037
facs on lipid accumulation
Sorted No BODIPY Gate
-1.3
N.D.
N.D.
-0.0
+0.2
growth on oleic acid
YNB Oleic Acid
-0.5
N.D.
N.D.
-0.1
-0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.5
N.D.
N.D.
-0.1
-0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.7
N.D.
N.D.
-0.1
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.6
N.D.
N.D.
-0.3
+0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.0
N.D.
N.D.
-0.3
-0.2
growth on oleic acid
YNB Glucose
-0.1
N.D.
N.D.
-0.2
-0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.5
N.D.
N.D.
+0.0
+0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.0
N.D.
N.D.
-0.1
-0.2
growth on ricinoleic acid
YNB Glucose
+0.1
N.D.
N.D.
-0.3
+0.0
ypd buoyancy
40 hrs Median Buoyancy
+0.0
N.D.
N.D.
+0.0
-0.1
ypd buoyancy
40 hrs Low Buoyancy
+0.3
N.D.
N.D.
-0.1
-0.1
auxotrophy
YNB Glucose
+0.3
N.D.
N.D.
+0.1
-0.3
growth on ypd
YPD
+0.3
N.D.
N.D.
-0.1
-0.0
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.2
N.D.
N.D.
+0.4
+0.1
auxotrophy
YNB Glucose 75mM Arginine
+0.5
N.D.
N.D.
-0.1
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+0.2
N.D.
N.D.
+0.1
+0.2
ypd buoyancy
40 hrs No Buoyancy Separation
+0.1
N.D.
N.D.
+0.1
+0.3
auxotrophy
YNB Glucose 75mM Methionine
+0.6
N.D.
N.D.
+0.0
-0.1
ypd buoyancy
40 hrs High Buoyancy
+0.8
N.D.
N.D.
+0.1
-0.2
auxotrophy
Dropout Complete
+0.8
N.D.
N.D.
+0.1
-0.1
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