Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15033 and 15034 are separated by 228 nucleotides15034 and 15035 are separated by 278 nucleotides15035 and 15036 are separated by 698 nucleotides15036 and 15037 are separated by 168 nucleotides mRNA_6665: 15033 - K01952 purL, PFAS phosphoribosylformylglycinamidine synthase, at 1,389,385 to 1,393,556 15033 mRNA_6666: 15034 - K18423 CSE1, CAS, XPO2 exportin-2 (importin alpha re-exporter), at 1,393,785 to 1,397,625 15034 mRNA_6667: 15035 - Hypothetical Protein, at 1,397,904 to 1,402,835 15035 mRNA_6668: 15036 - K03509 POLH DNA polymerase eta, at 1,403,534 to 1,406,837 15036 mRNA_6669: 15037 - K03564 BCP, PRXQ, DOT5 peroxiredoxin Q/BCP, at 1,407,006 to 1,408,136 15037
Group Condition mRNA_6665 mRNA_6666 mRNA_6667 mRNA_6668 mRNA_6669
15033 15034 15035 15036 15037
facs on lipid accumulation Sorted No BODIPY Gate -1.3 N.D. N.D. -0.0 +0.2
growth on oleic acid YNB Oleic Acid -0.5 N.D. N.D. -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 N.D. N.D. -0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 N.D. N.D. -0.3 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. -0.3 -0.2
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 N.D. N.D. +0.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. N.D. -0.1 -0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.3 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. N.D. +0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. N.D. -0.1 -0.1
auxotrophy YNB Glucose +0.3 N.D. N.D. +0.1 -0.3
growth on ypd YPD +0.3 N.D. N.D. -0.1 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. +0.4 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. N.D. -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. N.D. +0.1 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.6 N.D. N.D. +0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.8 N.D. N.D. +0.1 -0.2
auxotrophy Dropout Complete +0.8 N.D. N.D. +0.1 -0.1
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