Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15058 and 15059 are separated by 595 nucleotides15059 and 15060 are separated by 780 nucleotides15060 and 15061 are separated by 252 nucleotides15061 and 15062 are separated by 218 nucleotides mRNA_6690: 15058 - KOG4776 Uncharacterized conserved protein BCNT, at 1,450,331 to 1,454,298 15058 mRNA_6691: 15059 - KOG3010 Methyltransferase, at 1,454,894 to 1,456,156 15059 mRNA_6692: 15060 - KOG3888 Gamma-butyrobetaine,2-oxoglutarate dioxygenase, at 1,456,937 to 1,457,982 15060 mRNA_6693: 15061 - HMMPfam-Afadin- and alpha -actinin-Binding-PF11559, at 1,458,235 to 1,460,963 15061 mRNA_6694: 15062 - Hypothetical Protein, at 1,461,182 to 1,464,230 15062
Group Condition mRNA_6690 mRNA_6691 mRNA_6692 mRNA_6693 mRNA_6694
15058 15059 15060 15061 15062
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.6 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.2 N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.1 N.D. -0.3 N.D.
auxotrophy YNB Glucose +0.0 -0.4 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.3 N.D. +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.1 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.2 N.D. +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 N.D. +0.0 N.D.
growth on ypd YPD +0.0 -0.3 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.2 N.D. +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.1 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 N.D. +0.0 N.D.
growth on oleic acid YNB Glucose +0.0 -0.1 N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.1 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.1 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.3 N.D. +0.4 N.D.
auxotrophy Dropout Complete +0.0 -0.2 N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.3 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.2 N.D. +0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.2 N.D. +0.5 N.D.
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