Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15059 and 15060 are separated by 780 nucleotides15060 and 15061 are separated by 252 nucleotides15061 and 15062 are separated by 218 nucleotides15062 and 15063 are separated by 1364 nucleotides mRNA_6691: 15059 - KOG3010 Methyltransferase, at 1,454,894 to 1,456,156 15059 mRNA_6692: 15060 - KOG3888 Gamma-butyrobetaine,2-oxoglutarate dioxygenase, at 1,456,937 to 1,457,982 15060 mRNA_6693: 15061 - HMMPfam-Afadin- and alpha -actinin-Binding-PF11559, at 1,458,235 to 1,460,963 15061 mRNA_6694: 15062 - Hypothetical Protein, at 1,461,182 to 1,464,230 15062 mRNA_6695: 15063 - K01045 PON arylesterase / paraoxonase, at 1,465,595 to 1,467,254 15063
Group Condition mRNA_6691 mRNA_6692 mRNA_6693 mRNA_6694 mRNA_6695
15059 15060 15061 15062 15063
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. -0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. +0.1 N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.0 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. +0.0 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -0.1 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. -0.3 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.2 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.0 N.D. +0.0
growth on oleic acid YNB Glucose -0.1 N.D. +0.1 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.0 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.3 N.D. +0.4 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.1 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.2 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.1 N.D. +0.1
auxotrophy YNB Glucose -0.4 N.D. +0.2 N.D. +0.3
auxotrophy Dropout Complete -0.2 N.D. +0.4 N.D. +0.0
growth on ypd YPD -0.3 N.D. +0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. +0.5 N.D. -0.4
growth on ricinoleic acid YNB Glucose -0.1 N.D. +0.1 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.5 N.D. +0.1
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