Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15060 and 15061 are separated by 252 nucleotides15061 and 15062 are separated by 218 nucleotides15062 and 15063 are separated by 1364 nucleotides15063 and 15064 are separated by 319 nucleotides mRNA_6692: 15060 - KOG3888 Gamma-butyrobetaine,2-oxoglutarate dioxygenase, at 1,456,937 to 1,457,982 15060 mRNA_6693: 15061 - HMMPfam-Afadin- and alpha -actinin-Binding-PF11559, at 1,458,235 to 1,460,963 15061 mRNA_6694: 15062 - Hypothetical Protein, at 1,461,182 to 1,464,230 15062 mRNA_6695: 15063 - K01045 PON arylesterase / paraoxonase, at 1,465,595 to 1,467,254 15063 mRNA_6696: 15064 - KOG3519 Invasion-inducing protein TIAM1/CDC24 and related RhoGEF GTPases, at 1,467,574 to 1,471,494 15064
Group Condition mRNA_6692 mRNA_6693 mRNA_6694 mRNA_6695 mRNA_6696
15060 15061 15062 15063 15064
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 N.D. -0.3 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 N.D. +0.0 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. -0.5 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 N.D. -0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. -0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 N.D. +0.2 -0.1
growth on oleic acid YNB Glucose N.D. +0.1 N.D. -0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 N.D. +0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 N.D. +0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. -0.0 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.5 N.D. -0.4 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 N.D. +0.1 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 N.D. +0.3 +0.1
auxotrophy Dropout Complete N.D. +0.4 N.D. +0.0 -0.2
growth on oleic acid YNB Oleic Acid N.D. +0.4 N.D. -0.0 +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. +0.2 +0.0
auxotrophy YNB Glucose N.D. +0.2 N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.5 N.D. +0.1 -0.1
growth on ypd YPD N.D. +0.1 N.D. +0.4 +0.2
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