Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15064 and 15065 are separated by 540 nucleotides15065 and 15066 are separated by 350 nucleotides15066 and 15067 are separated by 378 nucleotides15067 and 15068 are separated by 341 nucleotides mRNA_6696: 15064 - KOG3519 Invasion-inducing protein TIAM1/CDC24 and related RhoGEF GTPases, at 1,467,574 to 1,471,494 15064 mRNA_6697: 15065 - K07188 LIPE, HSL hormone-sensitive lipase, at 1,472,035 to 1,475,787 15065 mRNA_6698: 15066 - K00819 rocD, OAT ornithine--oxo-acid transaminase, at 1,476,138 to 1,478,780 15066 mRNA_6699: 15067 - HMMPfam-Peptidase family M20/M25/M40-PF01546,HMMPfam-Peptidase dimerisation domain-PF07687,PIRSF--PIRSF037226,SUPERFAMILY--SSF53187,SUPERFAMILY--SSF55031,TIGRFAM-amidohydrolases amidohydrolase-TIGR01891, at 1,479,159 to 1,480,660 15067 mRNA_6700: 15068 - HMMPfam-Amidohydrolase family-PF07969,SUPERFAMILY--SSF51338,SUPERFAMILY--SSF51556, at 1,481,002 to 1,482,828 15068
Group Condition mRNA_6696 mRNA_6697 mRNA_6698 mRNA_6699 mRNA_6700
15064 15065 15066 15067 15068
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.3 -0.4 -1.2 +0.8
growth on ypd YPD +0.2 -0.0 -0.3 +0.0 -0.4
auxotrophy YNB Glucose -0.1 +0.1 -0.1 -0.5 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 +0.0 -0.4 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.1 -0.3 +0.4 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.1 -0.2 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 -0.2 -0.7 +0.4
growth on ricinoleic acid YNB Glucose +0.0 -0.1 +0.3 -0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.1 -0.7 -0.3 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.1 -0.5 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.2 -0.5 -0.5 +0.6
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.1 -0.5 +0.4 +0.2
growth on oleic acid YNB Oleic Acid +0.0 -0.2 +0.1 +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.2 +0.0 -0.3 +0.3
auxotrophy Dropout Complete -0.2 +0.2 -0.0 -0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.1 +0.0 -0.3 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.1 -0.2 +0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 -0.2 +0.4 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.0 +0.3 +0.8 -0.1
growth on oleic acid YNB Glucose -0.1 +0.1 +0.2 +0.4 +0.7
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.3 +0.4 +0.5 +0.3
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