Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15068 and 15069 are separated by 151 nucleotides15069 and 15070 are separated by 729 nucleotides15070 and 15071 are separated by 332 nucleotides15071 and 15072 are separated by 99 nucleotides mRNA_6700: 15068 - HMMPfam-Amidohydrolase family-PF07969,SUPERFAMILY--SSF51338,SUPERFAMILY--SSF51556, at 1,481,002 to 1,482,828 15068 mRNA_6701: 15069 - K08192 DAL MFS transporter, ACS family, allantoate permease, at 1,482,980 to 1,485,101 15069 mRNA_6702: 15070 - K08192 DAL MFS transporter, ACS family, allantoate permease, at 1,485,831 to 1,488,005 15070 mRNA_6703: 15071 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,488,338 to 1,490,366 15071 mRNA_6704: 15072 - HMMPfam-Peptidase family M20/M25/M40-PF01546,HMMPfam-Peptidase dimerisation domain-PF07687,SUPERFAMILY--SSF53187,SUPERFAMILY--SSF55031,TIGRFAM-amidohydrolases amidohydrolase-TIGR01891, at 1,490,466 to 1,492,340 15072
Group Condition mRNA_6700 mRNA_6701 mRNA_6702 mRNA_6703 mRNA_6704
15068 15069 15070 15071 15072
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.7 +0.1 +0.0 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -1.1 +0.2 -0.2 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.5 -0.2 -0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.5 -0.1 -0.0 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.2 +0.1 -0.6 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.0 -0.2 -0.5 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.0 -0.0 -0.1 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.5 -0.2 -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.6 -0.1 -0.0 -0.1
growth on ypd YPD -0.4 +0.4 -0.2 +0.1 -0.2
growth on ricinoleic acid YNB Glucose +0.1 +0.1 -0.1 -0.0 -0.2
auxotrophy YNB Glucose +0.1 +0.1 -0.0 +0.0 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.3 +0.1 -0.0 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.1 -0.2 +0.1 +0.3
growth on oleic acid YNB Oleic Acid +0.2 +0.0 -0.1 +0.2 -0.1
growth on oleic acid YNB Glucose +0.7 -0.2 -0.1 +0.0 -0.1
auxotrophy Dropout Complete +0.2 +0.3 -0.1 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 +0.1 -0.2 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.0 +0.0 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +0.2 -0.2 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 +0.8 +0.1 -0.2 -0.3
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