Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15069 and 15070 are separated by 729 nucleotides15070 and 15071 are separated by 332 nucleotides15071 and 15072 are separated by 99 nucleotides15072 and 15073 are separated by 260 nucleotides mRNA_6701: 15069 - K08192 DAL MFS transporter, ACS family, allantoate permease, at 1,482,980 to 1,485,101 15069 mRNA_6702: 15070 - K08192 DAL MFS transporter, ACS family, allantoate permease, at 1,485,831 to 1,488,005 15070 mRNA_6703: 15071 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,488,338 to 1,490,366 15071 mRNA_6704: 15072 - HMMPfam-Peptidase family M20/M25/M40-PF01546,HMMPfam-Peptidase dimerisation domain-PF07687,SUPERFAMILY--SSF53187,SUPERFAMILY--SSF55031,TIGRFAM-amidohydrolases amidohydrolase-TIGR01891, at 1,490,466 to 1,492,340 15072 mRNA_6705: 15073 - K11227 PBS2 mitogen-activated protein kinase kinase, at 1,492,601 to 1,495,154 15073
Group Condition mRNA_6701 mRNA_6702 mRNA_6703 mRNA_6704 mRNA_6705
15069 15070 15071 15072 15073
growth on ricinoleic acid YNB Ricinoleic Acid -1.1 +0.2 -0.2 -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.2 -0.2 -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 +0.1 +0.0 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.2 -0.5 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.6 -0.1 -0.0 -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.1 -0.6 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.5 -0.1 -0.0 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.5 -0.2 -0.1 +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.0 -0.1 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.2 -0.1 -0.1 N.D.
growth on oleic acid YNB Glucose -0.2 -0.1 +0.0 -0.1 N.D.
auxotrophy YNB Glucose +0.1 -0.0 +0.0 -0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.1 -0.0 -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.2 +0.1 +0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.0 -0.1 +0.2 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.1 -0.0 -0.2 N.D.
growth on ypd YPD +0.4 -0.2 +0.1 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.2 +0.1 +0.0 N.D.
auxotrophy Dropout Complete +0.3 -0.1 +0.1 -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.0 +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 +0.1 -0.2 -0.3 N.D.
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