Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15071 and 15072 are separated by 99 nucleotides15072 and 15073 are separated by 260 nucleotides15073 and 15074 are separated by 1860 nucleotides15074 and 15075 are separated by 199 nucleotides mRNA_6703: 15071 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,488,338 to 1,490,366 15071 mRNA_6704: 15072 - HMMPfam-Peptidase family M20/M25/M40-PF01546,HMMPfam-Peptidase dimerisation domain-PF07687,SUPERFAMILY--SSF53187,SUPERFAMILY--SSF55031,TIGRFAM-amidohydrolases amidohydrolase-TIGR01891, at 1,490,466 to 1,492,340 15072 mRNA_6705: 15073 - K11227 PBS2 mitogen-activated protein kinase kinase, at 1,492,601 to 1,495,154 15073 mRNA_6706: 15074 - K16261 YAT yeast amino acid transporter, at 1,497,015 to 1,499,228 15074 mRNA_6707: 15075 - KOG3750 Inositol phospholipid synthesis protein, Scs3p, at 1,499,428 to 1,503,895 15075
Group Condition mRNA_6703 mRNA_6704 mRNA_6705 mRNA_6706 mRNA_6707
15071 15072 15073 15074 15075
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.2 N.D. -1.2 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.4 N.D. -0.8 -0.4
auxotrophy YNB Glucose +0.0 -0.3 N.D. -1.0 -0.3
auxotrophy Dropout Complete +0.1 -0.1 N.D. -0.7 -0.4
growth on ypd YPD +0.1 -0.2 N.D. -0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.4 N.D. -0.4 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.0 N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.1 N.D. +0.5 -0.3
growth on oleic acid YNB Glucose +0.0 -0.1 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.3 N.D. +0.4 -0.1
growth on oleic acid YNB Oleic Acid +0.2 -0.1 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.0 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Glucose -0.0 -0.2 N.D. +0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.6 -0.1 N.D. +0.9 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. +0.3 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.0 N.D. -0.0 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 N.D. +0.3 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 N.D. +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.0 N.D. +0.8 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.3 N.D. +0.7 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.2 N.D. +1.0 -0.1
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