Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15083 and 15084 are separated by 265 nucleotides15084 and 15085 are separated by 415 nucleotides15085 and 15086 are separated by 1322 nucleotides15086 and 15087 are separated by 994 nucleotides mRNA_6715: 15083 - K13989 DERL2_3 Derlin-2/3, at 1,521,694 to 1,522,877 15083 mRNA_6716: 15084 - Hypothetical Protein, at 1,523,143 to 1,524,181 15084 mRNA_6717: 15085 - Hypothetical Protein, at 1,524,597 to 1,524,959 15085 mRNA_6718: 15086 - K13711 PI4K2 phosphatidylinositol 4-kinase type 2, at 1,526,282 to 1,529,015 15086 mRNA_6719: 15087 - Hypothetical Protein, at 1,530,010 to 1,530,905 15087
Group Condition mRNA_6715 mRNA_6716 mRNA_6717 mRNA_6718 mRNA_6719
15083 15084 15085 15086 15087
growth on oleic acid YNB Glucose -0.4 -0.6 N.D. -0.1 -0.1
growth on oleic acid YNB Oleic Acid -0.4 -0.5 N.D. +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.3 N.D. +0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.6 N.D. +0.2 +0.2
auxotrophy Dropout Complete -0.0 -0.6 N.D. +0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.4 N.D. +0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.4 N.D. +0.0 +0.0
auxotrophy YNB Glucose -0.0 -0.0 N.D. +0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.4 N.D. +0.4 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.3 N.D. +0.1 -0.4
growth on ypd YPD +0.0 -0.1 N.D. +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.1 N.D. +0.2 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.2 N.D. +0.2 +0.1
growth on ricinoleic acid YNB Glucose +0.1 +0.3 N.D. +0.2 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.5 N.D. +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.4 N.D. +0.3 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.4 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.6 N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.8 N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.9 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.8 N.D. +0.1 -0.2
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