Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15098 and 15099 are separated by 1573 nucleotides15099 and 15100 are separated by 503 nucleotides15100 and 15101 are separated by 1060 nucleotides15101 and 15102 are separated by 133 nucleotides mRNA_6730: 15098 - K00326 E1.6.2.2 cytochrome-b5 reductase, at 12,080 to 14,125 15098 mRNA_6731: 15099 - BLAST putative Ran-binding protein [Rhizoctonia solani 123E], at 15,699 to 18,601 15099 mRNA_6732: 15100 - KOG1530 Rhodanese-related sulfurtransferase, at 19,105 to 19,903 15100 mRNA_6733: 15101 - KOG4412 26S proteasome regulatory complex, subunit PSMD10, at 20,964 to 21,791 15101 mRNA_6734: 15102 - Hypothetical Protein, at 21,925 to 24,629 15102
Group Condition mRNA_6730 mRNA_6731 mRNA_6732 mRNA_6733 mRNA_6734
15098 15099 15100 15101 15102
growth on ypd YPD -0.0 +0.1 N.D. -0.3 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.1 N.D. -0.3 +0.2
auxotrophy Dropout Complete -0.3 -0.2 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 N.D. +0.1 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.1 N.D. -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.1 N.D. +0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.0 N.D. +0.1 -0.5
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.2 N.D. -0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.2 N.D. +0.0 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.1 N.D. +0.4 -0.5
auxotrophy YNB Glucose -0.2 -0.1 N.D. +0.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.0 N.D. +0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.4 N.D. -0.1 +0.2
growth on oleic acid YNB Glucose +0.0 -0.2 N.D. +0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.2 N.D. +0.4 +0.1
growth on oleic acid YNB Oleic Acid +0.2 +0.0 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.0 N.D. +0.3 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 N.D. +0.2 +0.1
growth on ricinoleic acid YNB Glucose +0.0 +0.1 N.D. +0.5 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.1 N.D. +0.4 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.3 N.D. +0.4 -0.2
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