Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15109 and 15110 are separated by 628 nucleotides15110 and 15111 are separated by 94 nucleotides15111 and 15112 are separated by 127 nucleotides15112 and 15113 are separated by 107 nucleotides mRNA_6741: 15109 - K01657 trpE anthranilate synthase component I, at 38,468 to 40,696 15109 mRNA_6742: 15110 - Hypothetical Protein, at 41,325 to 41,801 15110 mRNA_6743: 15111 - K06671 STAG1_2, SCC3, IRR1 cohesin complex subunit SA-1/2, at 41,896 to 46,673 15111 mRNA_6744: 15112 - KOG0813 Glyoxylase, at 46,801 to 48,312 15112 mRNA_6745: 15113 - HMMPfam-Uncharacterised conserved protein (DUF2315)-PF10231, at 48,420 to 49,672 15113
Group Condition mRNA_6741 mRNA_6742 mRNA_6743 mRNA_6744 mRNA_6745
15109 15110 15111 15112 15113
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.3 -0.5 -0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.5 -0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.7 -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.8 -0.0 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.8 +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.4 -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.3 +0.0 -0.1
growth on ypd YPD N.D. N.D. -1.0 +0.4 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.7 +0.2 +0.4
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.0 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.5 +0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.4 +0.1 +0.3
growth on oleic acid YNB Glucose N.D. N.D. -0.0 +0.1 +0.2
auxotrophy Dropout Complete N.D. N.D. -0.1 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.9 +0.7 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.3 +0.5 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.2 +0.1 +0.1
auxotrophy YNB Glucose N.D. N.D. -0.2 +0.3 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.7 +0.7 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +1.2 +0.1 +0.1
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