Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15112 and 15113 are separated by 107 nucleotides15113 and 15114 are separated by 117 nucleotides15114 and 15115 are separated by 146 nucleotides15115 and 15116 are separated by 487 nucleotides mRNA_6744: 15112 - KOG0813 Glyoxylase, at 46,801 to 48,312 15112 mRNA_6745: 15113 - HMMPfam-Uncharacterised conserved protein (DUF2315)-PF10231, at 48,420 to 49,672 15113 mRNA_6746: 15114 - KOG3381 Uncharacterized conserved protein, at 49,790 to 50,693 15114 mRNA_6747: 15115 - Hypothetical Protein, at 50,840 to 52,306 15115 mRNA_6748: 15116 - Hypothetical Protein, at 52,794 to 54,250 15116
Group Condition mRNA_6744 mRNA_6745 mRNA_6746 mRNA_6747 mRNA_6748
15112 15113 15114 15115 15116
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.8 N.D. -1.4 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.1 N.D. -0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.0 N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.3 N.D. -0.4 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.2 N.D. +0.0 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.1 N.D. -0.1 -0.0
auxotrophy Dropout Complete +0.1 +0.2 N.D. -0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 N.D. -0.0 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 N.D. +0.0 +0.0
auxotrophy YNB Glucose +0.3 +0.5 N.D. -0.4 -0.2
growth on ypd YPD +0.4 +0.4 N.D. -0.1 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.0 N.D. -0.1 +0.0
growth on oleic acid YNB Glucose +0.1 +0.2 N.D. -0.2 +0.3
growth on oleic acid YNB Oleic Acid -0.1 -0.1 N.D. +0.4 +0.1
growth on ricinoleic acid YNB Glucose +0.1 +0.2 N.D. -0.1 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 +0.1 N.D. -0.3 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.4 N.D. +0.3 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 N.D. +0.2 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.1 N.D. +0.4 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 +0.4 N.D. -0.3 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 +0.6 N.D. -0.2 +0.2
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