Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15118 and 15119 are separated by 77 nucleotides15119 and 15120 are separated by 219 nucleotides15120 and 15121 are separated by 1012 nucleotides15121 and 15122 are separated by 188 nucleotides mRNA_6750: 15118 - SUPERFAMILY--SSF47473, at 56,227 to 57,172 15118 mRNA_6751: 15119 - K10578 UBE2J1, NCUBE1, UBC6 ubiquitin-conjugating enzyme E2 J1, at 57,250 to 58,945 15119 mRNA_6752: 15120 - Hypothetical Protein, at 59,165 to 59,914 15120 mRNA_6753: 15121 - K00999 CDIPT CDP-diacylglycerol--inositol 3-phosphatidyltransferase, at 60,927 to 62,479 15121 mRNA_6754: 15122 - K15542 PFS2 polyadenylation factor subunit 2, at 62,668 to 65,062 15122
Group Condition mRNA_6750 mRNA_6751 mRNA_6752 mRNA_6753 mRNA_6754
15118 15119 15120 15121 15122
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.2 -1.0 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 -0.4 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.1 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.4 +0.1 N.D. N.D.
auxotrophy Dropout Complete +0.1 +0.0 -0.5 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.2 -0.4 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.1 -0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.2 -0.4 -0.0 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.2 +0.1 N.D. N.D.
auxotrophy YNB Glucose +0.2 -0.1 -0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.3 +0.3 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.0 -0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.0 +0.3 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.0 +0.2 N.D. N.D.
growth on ypd YPD -0.2 +0.3 +0.3 N.D. N.D.
growth on oleic acid YNB Glucose +0.3 -0.0 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.1 +0.5 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.5 +0.8 -0.7 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.1 +0.5 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.4 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.1 +0.5 N.D. N.D.
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