Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15122 and 15123 are separated by 157 nucleotides15123 and 15124 are separated by 121 nucleotides15124 and 15125 are separated by 420 nucleotides15125 and 15126 are separated by 803 nucleotides mRNA_6754: 15122 - K15542 PFS2 polyadenylation factor subunit 2, at 62,668 to 65,062 15122 mRNA_6755: 15123 - K14319 RANGAP1 Ran GTPase-activating protein 1, at 65,220 to 66,894 15123 mRNA_6756: 15124 - K02906 RP-L3, MRPL3, rplC large subunit ribosomal protein L3, at 67,016 to 68,273 15124 mRNA_6757: 15125 - K11838 USP7, UBP15 ubiquitin carboxyl-terminal hydrolase 7, at 68,694 to 73,091 15125 mRNA_6758: 15126 - HMMPfam-Beta-lactamase-PF00144,SUPERFAMILY--SSF56601, at 73,895 to 75,434 15126
Group Condition mRNA_6754 mRNA_6755 mRNA_6756 mRNA_6757 mRNA_6758
15122 15123 15124 15125 15126
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.8 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -1.4 +0.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.4 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.2 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.3 +0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.2 -0.0
auxotrophy YNB Glucose N.D. N.D. N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.4 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.0 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.4 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.0 +0.2
auxotrophy Dropout Complete N.D. N.D. N.D. +0.6 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +0.4 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.2 +0.3
growth on ypd YPD N.D. N.D. N.D. -0.1 +0.6
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.9 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.9 -0.2
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