Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15130 and 15131 are separated by 757 nucleotides15131 and 15132 are separated by 1230 nucleotides15132 and 15133 are separated by 181 nucleotides15133 and 15134 are separated by 125 nucleotides mRNA_6762: 15130 - K02942 RP-LP1, RPLP1 large subunit ribosomal protein LP1, at 80,417 to 80,968 15130 mRNA_6763: 15131 - K11584 PPP2R5 serine/threonine-protein phosphatase 2A regulatory subunit B', at 81,726 to 84,068 15131 mRNA_6764: 15132 - K12273 SEC66 translocation protein SEC66, at 85,299 to 86,536 15132 mRNA_6765: 15133 - HMMPfam-GDSL-like Lipase/Acylhydrolase family-PF13472,SUPERFAMILY--SSF52266, at 86,718 to 88,049 15133 mRNA_6766: 15134 - HMMPfam-PIG-X / PBN1-PF08320,SMART-PIG-X / PBN1-SM00780, at 88,175 to 88,997 15134
Group Condition mRNA_6762 mRNA_6763 mRNA_6764 mRNA_6765 mRNA_6766
15130 15131 15132 15133 15134
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.6 N.D. +0.1 N.D.
auxotrophy Dropout Complete N.D. -0.3 N.D. -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.8 N.D. +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 N.D. +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 N.D. -0.3 N.D.
growth on oleic acid YNB Glucose N.D. -0.0 N.D. -0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 N.D. +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. -0.0 N.D.
growth on ypd YPD N.D. +0.0 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.6 N.D. -0.3 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.6 N.D. -0.2 N.D.
auxotrophy YNB Glucose N.D. +0.1 N.D. +0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.5 N.D. -0.0 N.D.
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