Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15133 and 15134 are separated by 125 nucleotides15134 and 15135 are separated by 123 nucleotides15135 and 15136 are separated by 180 nucleotides15136 and 15137 are separated by 310 nucleotides mRNA_6765: 15133 - HMMPfam-GDSL-like Lipase/Acylhydrolase family-PF13472,SUPERFAMILY--SSF52266, at 86,718 to 88,049 15133 mRNA_6766: 15134 - HMMPfam-PIG-X / PBN1-PF08320,SMART-PIG-X / PBN1-SM00780, at 88,175 to 88,997 15134 mRNA_6767: 15135 - KOG0393 Ras-related small GTPase, Rho type, at 89,121 to 90,132 15135 mRNA_6768: 15136 - HMMPfam-DNA repair protein endonuclease SAE2/CtIP C-terminus-PF08573, at 90,313 to 94,205 15136 mRNA_6769: 15137 - K01953 asnB, ASNS asparagine synthase (glutamine-hydrolysing), at 94,516 to 96,583 15137
Group Condition mRNA_6765 mRNA_6766 mRNA_6767 mRNA_6768 mRNA_6769
15133 15134 15135 15136 15137
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. N.D. -0.9 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 N.D. N.D. -1.6 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. N.D. -0.5 N.D.
auxotrophy Dropout Complete -0.1 N.D. N.D. -0.7 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. -0.8 N.D.
growth on ricinoleic acid YNB Glucose -0.2 N.D. N.D. -0.4 N.D.
growth on ypd YPD +0.2 N.D. N.D. -0.8 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. N.D. -0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. N.D. -0.5 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. N.D. +0.1 N.D.
growth on oleic acid YNB Glucose -0.0 N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.1 N.D.
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. +0.4 N.D.
auxotrophy YNB Glucose +0.4 N.D. N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. +0.5 N.D.
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