Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15135 and 15136 are separated by 180 nucleotides15136 and 15137 are separated by 310 nucleotides15137 and 15138 are separated by 258 nucleotides15138 and 15139 are separated by 965 nucleotides mRNA_6767: 15135 - KOG0393 Ras-related small GTPase, Rho type, at 89,121 to 90,132 15135 mRNA_6768: 15136 - HMMPfam-DNA repair protein endonuclease SAE2/CtIP C-terminus-PF08573, at 90,313 to 94,205 15136 mRNA_6769: 15137 - K01953 asnB, ASNS asparagine synthase (glutamine-hydrolysing), at 94,516 to 96,583 15137 mRNA_6770: 15138 - K03350 APC3, CDC27 anaphase-promoting complex subunit 3, at 96,842 to 100,028 15138 mRNA_6771: 15139 - Hypothetical Protein, at 100,994 to 101,449 15139
Group Condition mRNA_6767 mRNA_6768 mRNA_6769 mRNA_6770 mRNA_6771
15135 15136 15137 15138 15139
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.6 N.D. N.D. -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 N.D. N.D. -1.9
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. N.D. -1.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.8 N.D. N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 N.D. N.D. -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.5 N.D. N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 N.D. N.D. -0.4
growth on ricinoleic acid YNB Glucose N.D. -0.4 N.D. N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.9 N.D. N.D. +0.7
auxotrophy Dropout Complete N.D. -0.7 N.D. N.D. +0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.4 N.D. N.D. -0.6
growth on oleic acid YNB Glucose N.D. -0.1 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.5 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.5 N.D. N.D. +1.0
growth on oleic acid YNB Oleic Acid N.D. +0.4 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 N.D. N.D. +0.5
auxotrophy YNB Glucose N.D. -0.2 N.D. N.D. +1.7
growth on ypd YPD N.D. -0.8 N.D. N.D. +3.1
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