Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15140 and 15141 are separated by 359 nucleotides15141 and 15142 are separated by 169 nucleotides15142 and 15143 are separated by 143 nucleotides15143 and 15144 are separated by 1221 nucleotides mRNA_6772: 15140 - KOG0202 Ca2+ transporting ATPase, at 102,291 to 102,787 15140 mRNA_6773: 15141 - HMMPfam-Cation transporter/ATPase, N-terminus-PF00690,SUPERFAMILY--SSF81665, at 103,147 to 103,439 15141 mRNA_6774: 15142 - HMMPfam-Mitochondrial ribosomal protein L31-PF09784, at 103,609 to 104,047 15142 mRNA_6775: 15143 - K14784 CMS1 protein CMS1, at 104,191 to 105,444 15143 mRNA_6776: 15144 - KOG1183 N-acetylglucosaminyltransferase complex, subunit PIG-Q/GPI1, required for phosphatidylinositol biosynthesis, at 106,666 to 108,156 15144
Group Condition mRNA_6772 mRNA_6773 mRNA_6774 mRNA_6775 mRNA_6776
15140 15141 15142 15143 15144
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. -2.1 +0.2
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -2.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.8 N.D. N.D. -1.3 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. N.D. -2.0 +0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.7 N.D. N.D. -0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. N.D. -0.6 +0.3
growth on ricinoleic acid YNB Glucose +0.2 N.D. N.D. -1.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 N.D. N.D. -0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. N.D. +0.7 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 N.D. N.D. -0.0 -0.3
ypd buoyancy 40 hrs Low Buoyancy +1.0 N.D. N.D. -0.3 -0.3
growth on ypd YPD +1.1 N.D. N.D. -0.8 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 N.D. N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. N.D. +0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. +0.8 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.7 N.D. N.D. +1.9 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.6 N.D. N.D. +0.5 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. N.D. +1.3 -0.3
auxotrophy YNB Glucose +0.2 N.D. N.D. +0.9 +0.3
auxotrophy Dropout Complete -1.4 N.D. N.D. +3.0 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. N.D. +1.9 +0.1
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