Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15141 and 15142 are separated by 169 nucleotides15142 and 15143 are separated by 143 nucleotides15143 and 15144 are separated by 1221 nucleotides15144 and 15145 are separated by 286 nucleotides mRNA_6773: 15141 - HMMPfam-Cation transporter/ATPase, N-terminus-PF00690,SUPERFAMILY--SSF81665, at 103,147 to 103,439 15141 mRNA_6774: 15142 - HMMPfam-Mitochondrial ribosomal protein L31-PF09784, at 103,609 to 104,047 15142 mRNA_6775: 15143 - K14784 CMS1 protein CMS1, at 104,191 to 105,444 15143 mRNA_6776: 15144 - KOG1183 N-acetylglucosaminyltransferase complex, subunit PIG-Q/GPI1, required for phosphatidylinositol biosynthesis, at 106,666 to 108,156 15144 mRNA_6777: 15145 - Hypothetical Protein, at 108,443 to 109,222 15145
Group Condition mRNA_6773 mRNA_6774 mRNA_6775 mRNA_6776 mRNA_6777
15141 15142 15143 15144 15145
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -2.0 +0.5 -1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.6 +0.3 -1.5
growth on oleic acid YNB Oleic Acid N.D. N.D. -2.1 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.0 -0.3 -1.2
growth on oleic acid YNB Glucose N.D. N.D. -2.0 +0.2 +0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.4 +0.1 -0.6
growth on ypd YPD N.D. N.D. -0.8 +0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.2 -0.4 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 +0.0 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -1.3 +0.7 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +1.3 -0.3 -1.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.2 +0.2 -0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. -1.1 +0.3 +0.7
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.3 -0.3 +0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.7 -0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.8 +0.0 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.5 +0.0 +0.1
auxotrophy YNB Glucose N.D. N.D. +0.9 +0.3 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +1.9 -0.2 +0.1
auxotrophy Dropout Complete N.D. N.D. +3.0 -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +1.9 +0.1 +0.9
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