Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15172 and 15173 are separated by 118 nucleotides15173 and 15174 are separated by 655 nucleotides15174 and 15175 are separated by 423 nucleotides15175 and 15176 are separated by 586 nucleotides mRNA_6804: 15172 - HMMPfam-CFEM domain-PF05730, at 173,731 to 174,873 15172 mRNA_6805: 15173 - Hypothetical Protein, at 174,992 to 176,183 15173 mRNA_6806: 15174 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 176,839 to 177,908 15174 mRNA_6807: 15175 - HMMPfam-GRIP domain-PF01465,ProSiteProfiles-GRIP domain profile.-PS50913,SMART-golgin-97, RanBP2alpha,Imh1p and p230/golgin-245-SM00755, at 178,332 to 182,273 15175 mRNA_6808: 15176 - K12856 PRPF8, PRP8 pre-mRNA-processing factor 8, at 182,860 to 191,504 15176
Group Condition mRNA_6804 mRNA_6805 mRNA_6806 mRNA_6807 mRNA_6808
15172 15173 15174 15175 15176
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.3 N.D. -0.4 -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.0 N.D. +0.1 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.0 N.D. +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.1 N.D. -0.0 +0.0
auxotrophy YNB Glucose +0.4 +0.1 N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.3 N.D. +0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.2 N.D. +0.0 -0.6
growth on ypd YPD -0.2 +0.1 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.3 N.D. +0.2 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.6 N.D. -0.1 +1.0
growth on ricinoleic acid YNB Glucose +0.1 +0.3 N.D. +0.2 -0.2
auxotrophy Dropout Complete +0.2 +0.1 N.D. +0.0 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.2 N.D. -0.2 +0.8
growth on oleic acid YNB Glucose -0.1 +0.0 N.D. +0.3 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 N.D. +0.0 +0.7
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 N.D. +0.0 +0.6
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.2 N.D. +0.1 +0.8
growth on oleic acid YNB Oleic Acid +0.0 +0.0 N.D. +0.4 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.1 N.D. +0.7 +0.4
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.3 N.D. +0.4 +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.4 N.D. +0.7 +0.2
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