Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15176 and 15177 are separated by 367 nucleotides15177 and 15178 are separated by 352 nucleotides15178 and 15179 are separated by 241 nucleotides15179 and 15180 are separated by 284 nucleotides mRNA_6808: 15176 - K12856 PRPF8, PRP8 pre-mRNA-processing factor 8, at 182,860 to 191,504 15176 mRNA_6809: 15177 - HMMPfam-F-box-like-PF12937,SUPERFAMILY--SSF52047,SUPERFAMILY--SSF81383, at 191,872 to 193,044 15177 mRNA_6810: 15178 - HMMPfam-Acetyltransferase (GNAT) family-PF00583,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 193,397 to 194,230 15178 mRNA_6811: 15179 - K03953 NDUFA9 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 9, at 194,472 to 196,083 15179 mRNA_6812: 15180 - K00074 paaH, hbd, fadB, mmgB 3-hydroxybutyryl-CoA dehydrogenase, at 196,368 to 197,656 15180
Group Condition mRNA_6808 mRNA_6809 mRNA_6810 mRNA_6811 mRNA_6812
15176 15177 15178 15179 15180
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.5 -0.4 N.D. -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.5 -0.2 N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.2 -0.6 +0.0 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.4 -0.5 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.6 +0.5 -0.1 N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 +0.2 -0.1 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.5 +0.3 -0.3 N.D. +0.1
auxotrophy YNB Glucose -0.3 -0.2 +0.5 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.0 -0.6 -0.5 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.6 +0.2 N.D. +0.1
growth on ypd YPD +0.2 -0.2 -0.0 N.D. -0.0
growth on oleic acid YNB Glucose +0.4 -0.3 +0.1 N.D. -0.1
auxotrophy Dropout Complete +0.0 -0.3 +0.6 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.7 -0.1 -0.4 N.D. +0.0
growth on oleic acid YNB Oleic Acid +0.6 -0.2 +0.1 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.1 +0.2 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 -0.3 -0.4 N.D. +0.4
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.3 +0.3 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.8 +0.1 -0.4 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.7 -0.0 -0.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine +0.6 -0.1 +0.4 N.D. +0.1
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