Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15224 and 15225 are separated by 1266 nucleotides15225 and 15226 are separated by 934 nucleotides15226 and 15227 are separated by 953 nucleotides15227 and 15228 are separated by 594 nucleotides mRNA_6856: 15224 - K10395 KIF4_21_27 kinesin family member 4/21/27, at 284,062 to 291,229 15224 mRNA_6857: 15225 - Hypothetical Protein, at 292,496 to 294,098 15225 mRNA_6858: 15226 - SUPERFAMILY--SSF103473, at 295,033 to 296,248 15226 mRNA_6859: 15227 - K16794 PAFAH1B1, LIS1 platelet-activating factor acetylhydrolase IB subunit alpha, at 297,202 to 298,993 15227 mRNA_6860: 15228 - K07513 ACAA1 acetyl-CoA acyltransferase 1, at 299,588 to 301,362 15228
Group Condition mRNA_6856 mRNA_6857 mRNA_6858 mRNA_6859 mRNA_6860
15224 15225 15226 15227 15228
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.1 -0.4 N.D. -0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.1 -0.4 N.D. -0.4
growth on oleic acid YNB Oleic Acid -0.0 -0.3 -0.3 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.0 -0.4 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.1 +0.0 N.D. -0.2
auxotrophy Dropout Complete +0.2 -0.3 -0.5 N.D. +0.5
growth on ricinoleic acid YNB Glucose +0.1 -0.1 +0.0 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.1 -0.3 N.D. +0.4
auxotrophy YNB Glucose +0.2 +0.1 -0.5 N.D. +0.3
growth on ypd YPD +0.1 -0.3 -0.1 N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.1 +0.1 N.D. -0.1
growth on oleic acid YNB Glucose +0.1 -0.3 -0.2 N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.4 +0.4 N.D. -0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.2 -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.2 +0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.4 +0.1 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.0 +0.4 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.1 +0.1 N.D. +0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.3 -0.0 N.D. +0.8
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.4 +0.2 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.4 +0.3 N.D. +0.2
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