Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15238 and 15239 are separated by 179 nucleotides15239 and 15240 are separated by 175 nucleotides15240 and 15241 are separated by 230 nucleotides15241 and 15242 are separated by 80 nucleotides mRNA_6870: 15238 - K08736 MSH3 DNA mismatch repair protein MSH3, at 315,464 to 319,765 15238 mRNA_6871: 15239 - K17600 VPS54 vacuolar protein sorting-associated protein 54, at 319,945 to 325,501 15239 mRNA_6872: 15240 - KOG3035 Isoamyl acetate-hydrolyzing esterase, at 325,677 to 326,693 15240 mRNA_6873: 15241 - K01409 KAE1, tsaD, QRI7 N6-L-threonylcarbamoyladenine synthase, at 326,924 to 328,423 15241 mRNA_6874: 15242 - BLAST similar to An13g00570 [Aspergillus niger], at 328,504 to 329,604 15242
Group Condition mRNA_6870 mRNA_6871 mRNA_6872 mRNA_6873 mRNA_6874
15238 15239 15240 15241 15242
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.5 -1.7 -0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -1.0 +0.4 +0.0 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.4 -0.5 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.1 -0.7 -0.1 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 +0.0 +0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.1 +0.3 -0.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 -0.3 +0.0 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.2 -0.0 +0.1 +0.1
growth on ricinoleic acid YNB Glucose +0.0 +0.2 -0.3 +0.0 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 +0.5 +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.2 -0.1 +0.4 +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 +0.2 +0.2 +0.3
growth on ypd YPD +0.1 +0.2 +0.1 +1.0 -0.3
auxotrophy Dropout Complete +0.2 +0.5 -0.2 +0.4 +0.1
growth on oleic acid YNB Oleic Acid +0.2 +0.6 -0.2 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.0 +1.0 -0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.4 -0.2 +0.5 +0.4
growth on oleic acid YNB Glucose +0.0 +0.2 +0.7 +0.1 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.5 +0.8 +0.2 +0.1
auxotrophy YNB Glucose +0.3 +0.3 +0.2 +0.4 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.4 +1.4 +0.4 -0.7
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