Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15251 and 15252 are separated by 281 nucleotides15252 and 15253 are separated by 102 nucleotides15253 and 15254 are separated by 1312 nucleotides15254 and 15255 are separated by 200 nucleotides mRNA_6883: 15251 - BLAST similar to An03g02750 [Aspergillus kawachii IFO 4308], at 348,850 to 350,156 15251 mRNA_6884: 15252 - K00943 tmk, DTYMK dTMP kinase, at 350,438 to 351,511 15252 mRNA_6885: 15253 - KOG0565 Inositol polyphosphate 5-phosphatase and related proteins, at 351,614 to 354,932 15253 mRNA_6886: 15254 - K09493 CCT1, TCP1 T-complex protein 1 subunit alpha, at 356,245 to 358,443 15254 mRNA_6887: 15255 - KOG2793 Putative N2,N2-dimethylguanosine tRNA methyltransferase, at 358,644 to 359,526 15255
Group Condition mRNA_6883 mRNA_6884 mRNA_6885 mRNA_6886 mRNA_6887
15251 15252 15253 15254 15255
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. -0.3 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 N.D. -0.0 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.1 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.1 N.D. -0.0
auxotrophy YNB Glucose +0.4 N.D. +0.2 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. +0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.1 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. +0.0 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. -0.3 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. +0.1 N.D. +0.0
growth on ypd YPD +0.3 N.D. -0.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. -0.0 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.6 N.D. +0.0 N.D. +0.0
auxotrophy Dropout Complete +0.5 N.D. +0.1 N.D. +0.1
growth on ricinoleic acid YNB Glucose +1.1 N.D. +0.3 N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 N.D. +0.1 N.D. -0.0
growth on oleic acid YNB Glucose +0.7 N.D. +0.2 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate +1.3 N.D. +0.1 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +2.4 N.D. -0.5 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +2.0 N.D. +0.1 N.D. +0.0
growth on oleic acid YNB Oleic Acid +1.3 N.D. +0.3 N.D. +0.5
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